FastQCFastQC Report
Mon 6 Jun 2016
ERR523044_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523044_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8865481
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT203810.2298916437810876No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT168590.1901645268880504No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93080.10499148325962233No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA122150.050.5783421
GTATCAA215200.040.712781
TCAACGC259200.032.93984
ATCAACG262450.032.5891083
CAACGCA263300.032.426695
TATCAAC276600.031.2486522
AACGCAG274350.031.2186376
ACGCAGA314300.027.1156627
CGCAGAG326950.026.0215118
GTACATG279550.025.7360171
TACATGG287900.024.0960832
ACATGGG290850.023.3478263
GCAGAGT372000.022.5030069
CATGGGG190650.021.5168384
GTGGTAT69100.020.6327881
CAGAGTA360950.019.77536410-11
ATGGGGG107150.019.602615
AGAGTAC333550.019.53378510-11
GAGTACT217800.019.3049712-13
TGGTATC74750.017.7312622
Produced by FastQC (version 0.11.5)