FastQCFastQC Report
Mon 6 Jun 2016
ERR523044_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523044_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8865481
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT187320.21129141216364908No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163660.18460363289933168No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA121050.051.614411
GTATCAA214450.041.179011
TCAACGC261050.033.358514
ATCAACG265600.032.770833
CAACGCA266750.032.6280755
AACGCAG276300.031.5338066
TATCAAC281700.030.9722082
ACGCAGA315550.027.521937
CGCAGAG328800.026.3751758
GTACATG279000.025.3281481
TACATGG287850.024.0032842
GCAGAGT371900.023.2806079
ACATGGG294000.022.7793873
CATGGGG189300.020.804074
GAGTACT216600.020.38831912-13
CAGAGTA363350.020.13560110-11
AGAGTAC339100.019.97454310-11
GTGGTAT68650.018.7093981
GTACTTT232700.018.6729614-15
ATGGGGG104050.017.117965
Produced by FastQC (version 0.11.5)