FastQCFastQC Report
Mon 6 Jun 2016
ERR523043_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523043_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2311605
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88570.3831536962413561No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69220.2994456232790637No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39560.17113650472290898No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23680.10243964691199405No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG33850.051.684922
ACCTGGG36950.046.4587363
TATAACG2900.043.7870182
ATAACGC2950.043.045793
GGTATCA59350.038.194121
TAACGCA3550.037.095284
GTACCTG50800.035.3646851
GTATCAA90700.031.9406381
CCTGGGG35250.031.7546124
TATCACG2051.8189894E-1229.8242192
TCAACGC105350.027.2322964
TATCAAC107600.026.8808122
CAACGCA106800.026.7733385
ATCAACG107150.026.6431473
AACGCAG110650.026.0430036
GTATAAC7250.025.9472981
GTATAGC7700.025.6524431
GTACCGG4150.024.9312881
GTACATG83900.024.1593631
TACATGG84300.023.3757742
Produced by FastQC (version 0.11.5)