FastQCFastQC Report
Mon 6 Jun 2016
ERR523043_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523043_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2311605
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80720.3491946072101419No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT74840.32375773542625147No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70840.30645374101544165No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39240.1697521851700442No Hit
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT36690.15872088873315293RNA PCR Primer, Index 39 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG36700.053.5426562
ACCTGGG39150.048.8700683
TATAACG2850.041.236792
CCTGGGG31550.037.9945374
GTACCTG53100.037.6549871
TAACGCA3050.035.4493524
ATAACGC3050.033.9080733
GGTATCA66200.032.1224441
GTATCAA97250.030.4291041
TCAACGC111000.026.0454884
CAACGCA111850.025.9321755
ATCAACG112050.025.8853243
TATCAAC113500.025.6380212
TACCGGG2409.094947E-1225.4637182
CTGGGGG20450.025.0566435
AACGCAG117250.024.8923036
TGGGGGG34800.023.229276
GTACATG86900.023.0631141
TACATGG87500.022.4573232
ACGCAGA131200.022.3177267
Produced by FastQC (version 0.11.5)