FastQCFastQC Report
Mon 6 Jun 2016
ERR523042_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523042_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3740116
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32400.039.351671
GTATCAA51600.035.103461
TCAACGC55150.031.186174
CAACGCA56900.030.3091965
ATCAACG57300.030.2640693
AACGCAG61650.027.8155296
TATCAAC64050.027.518942
GTACATG124000.025.9521481
TACATGG125000.025.1180572
ACATGGG132800.022.9315013
ACGCAGA75550.022.5735427
CGCAGAG81100.021.1429048
CATGGGG98450.021.0021364
ATGGGGG52850.018.4943495
TATACCG2403.4283348E-617.6218685
GTATAGG10500.017.474881
AGTACAT94050.017.4415972
GAGTACA99050.017.432131
GCAGAGT96650.017.3523779
GGACCGT5700.016.4847626
Produced by FastQC (version 0.11.5)