FastQCFastQC Report
Mon 6 Jun 2016
ERR523042_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523042_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3740116
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA48650.038.6828461
GGTATCA30900.036.0853651
TCAACGC55450.032.205894
CAACGCA56200.031.7760965
ATCAACG58300.030.7133433
TATCAAC60600.029.7258152
AACGCAG61350.029.1848776
GTACATG127150.026.5674171
TACATGG129650.025.3215622
ACGCAGA74050.024.0519337
ACATGGG134950.023.4375863
CATGGGG97850.021.9006634
GTATACG1954.6341847E-721.7144661
CGCAGAG82000.021.6662358
ATGGGGG50550.018.1287025
GAGTACA98350.017.891161
GTATAGG10100.017.7012311
GCAGAGT97950.017.5623329
AGTACAT94100.016.994052
GTATTAA15050.016.255791
Produced by FastQC (version 0.11.5)