FastQCFastQC Report
Mon 6 Jun 2016
ERR523041_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523041_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5991812
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT206320.3443365713076445No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161130.2689169820414926No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100520.16776227291510482No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA96200.049.944741
TACCTGG65050.043.137352
GTATCAA174050.041.718721
ACCTGGG78650.034.8977133
GTACCTG84350.034.3664551
TCAACGC210950.033.8181344
ATCAACG214100.033.300573
CAACGCA214400.033.229285
TATCAAC220750.032.620042
AACGCAG223000.032.320636
GTACATG189000.029.2062171
ACGCAGA251700.028.5421857
TACATGG194150.027.913762
CGCAGAG260700.027.5556878
ACATGGG199000.026.5458153
CATGGGG140750.026.310874
TATCACG6000.025.85172
GCAGAGT288050.024.807169
TATAACG5950.024.4890042
ATGGGGG95200.023.7934045
Produced by FastQC (version 0.11.5)