FastQCFastQC Report
Mon 6 Jun 2016
ERR523041_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523041_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5991812
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT188640.31482963751199133No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158710.26487813703100166No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97110.16207117312759478No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA96400.050.368151
TACCTGG71100.039.693122
GTATCAA168450.039.3543931
TATAACG4450.034.8808822
ACCTGGG84400.032.35013
GTACCTG89850.032.2040371
TCAACGC205800.031.739534
ATCAACG208750.031.2467563
CAACGCA210450.031.082895
TATCAAC213850.030.7930242
AACGCAG221700.029.7380286
GTACATG189600.029.256931
TACATGG195500.027.8849722
ACATGGG197750.026.7110983
ATGGGGG90200.026.5701925
ACGCAGA250250.026.3453397
CATGGGG139750.025.9258884
CGCAGAG259450.025.3635798
TATCACG5500.023.0905672
CCTGGGG79300.022.9926824
Produced by FastQC (version 0.11.5)