FastQCFastQC Report
Mon 6 Jun 2016
ERR523040_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523040_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5019978
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT238260.47462359396794174No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT194330.38711325029711285No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118080.23522015435127405No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115130.22934363457369733No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54200.10796860065920608No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG104300.057.6234662
GTACCTG118250.051.6798861
ACCTGGG115900.050.6799473
TATAACG7050.041.3576052
CCTGGGG97650.039.8703544
ATAACGC7600.038.9837953
TAACGCA8850.034.0039024
GGTATCA164550.032.9357951
GTATCAA245650.030.0291351
CTGGGGG60200.029.4452445
TATCACG4450.026.422
GTACCCG10250.026.1616121
TCAACGC289850.025.3558224
CAACGCA293350.025.0520465
TATCAAC294300.024.9759222
ATCAACG294550.024.9070763
TGGGGGG101250.024.8292886
CATGGGG133750.024.6442874
AACGCAG304600.024.3589066
GTACATG242000.023.4836241
Produced by FastQC (version 0.11.5)