FastQCFastQC Report
Mon 6 Jun 2016
ERR523040_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523040_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5019978
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT215050.4283883315823297No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT186670.3718542192814391No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112090.2232878311418895No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100490.2001801601520963No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG106450.060.4023632
GTACCTG120250.054.198391
ACCTGGG120350.052.636113
CCTGGGG100200.042.585144
TATAACG6750.041.0829622
TAACGCA7750.038.201364
ATAACGC8350.034.3307533
CTGGGGG58450.033.4463545
GGTATCA161300.033.1170921
TATCACG5200.029.8279972
GTATCAA234350.029.7968081
GTACCCG10000.027.2732091
TGGGGGG88900.025.3734466
TCAACGC276750.024.927484
CAACGCA279450.024.7034515
ATCAACG279600.024.6061613
TATCAAC281100.024.5458852
AACGCAG288950.024.0376346
GTATAAC15750.023.5860271
GTACATG230000.022.1620371
Produced by FastQC (version 0.11.5)