FastQCFastQC Report
Mon 6 Jun 2016
ERR523039_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523039_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9096218
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT642740.7066013589384071No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT295590.32495923030868434No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT230250.25312717879013014No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT194850.21420990569926973No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC152910.16810283130857243No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC137000.1506120455776236No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130670.14365310945713922No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA116010.12753652122233658No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG343500.025.6525921
TACATGG349550.024.6687562
ACATGGG353550.023.5128573
GAGTACT254950.021.1918112-13
CATGGGG223600.020.941784
AGAGTAC419550.019.54356810-11
GTACTTT277700.019.18624314-15
AGTACTT270950.018.27581212-13
GAGTACA271600.017.2258911
CATGGGA139700.016.8267274
AGTACAT269650.016.1635422
ATGGGGG111400.016.0369595
ACTTTTT342850.015.84780416-17
TACTTTT327400.015.08979714-15
GTGTAGC50900.014.8729291
GTATATG39650.014.4678851
GTATAGG27950.013.9631911
GTATTAG29150.013.7109891
ATGGGGA100900.013.6987145
TATACTG42350.013.6544875
Produced by FastQC (version 0.11.5)