FastQCFastQC Report
Mon 6 Jun 2016
ERR523039_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523039_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9096218
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT651680.7164296194308447No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT274830.30213655829268826No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220520.2424304254801281No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT192100.21118667120774812No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC158250.17397340301210898No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC140510.1544707921468021No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124830.13723285875514415No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA120760.13275847170769214No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA95700.10520856030495312No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG333500.025.7061
TACATGG339450.024.7425252
ACATGGG348450.023.5344943
GAGTACT262300.021.1580312-13
CATGGGG216050.020.9252514
AGAGTAC422450.019.89733710-11
GTACTTT278550.019.60814314-15
AGTACTT279550.018.4314112-13
ATGGGGG104050.017.2981415
GAGTACA265950.016.9221521
ACTTTTT337450.016.33631316-17
CATGGGA136900.016.2713954
TACTTTT326700.015.64637114-15
AGTACAT261200.015.5466152
GTATAGG28550.015.4833671
TATACTG41950.014.7871575
ATGGGGA98300.014.724485
GTGTAGC54400.014.1771381
GTATAGC30500.013.7225191
GTATTAC23650.013.7202361
Produced by FastQC (version 0.11.5)