FastQCFastQC Report
Mon 6 Jun 2016
ERR523038_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523038_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5729099
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT223540.3901835175129632No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT179730.31371425070504105No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99530.17372714278458096No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG75200.041.7097662
ACCTGGG85800.035.843683
GTACCTG88950.035.7383351
GGTATCA152950.033.635771
GTATCAA237000.032.69961
TATAACG5350.029.006142
TCAACGC274450.027.9627024
ATCAACG279600.027.4812873
CAACGCA280200.027.3541135
TATCAAC285350.026.878552
AACGCAG289950.026.6195966
GTACATG218650.024.4536971
CCTGGGG82550.024.4378174
ACGCAGA323500.023.7403727
TACATGG223800.023.3654672
CGCAGAG333350.023.0239728
ACATGGG223850.022.6245943
CATGGGG142100.022.5690084
TAACGCA7350.021.752734
ATAACGC7450.021.4607473
Produced by FastQC (version 0.11.5)