FastQCFastQC Report
Mon 6 Jun 2016
ERR523038_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523038_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5729099
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT206850.3610515370741542No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT178650.31182913753104985No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97770.1706551065010397No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG73050.041.6923832
ACCTGGG84350.035.493183
GTACCTG87500.035.3134271
GGTATCA149250.034.851651
GTATCAA227700.031.973521
TATAACG5450.031.0461642
TCAACGC265550.026.9022034
ATCAACG271050.026.3389783
CAACGCA271400.026.288615
TATCAAC276200.026.0016922
AACGCAG282850.025.372656
CCTGGGG80300.025.2262254
ATAACGC7250.024.6340683
GTACATG212550.023.9413531
TAACGCA7500.023.1862754
TACATGG219250.023.0446852
ACGCAGA320550.022.418287
ACATGGG218150.021.8676153
CGCAGAG329850.021.7003338
CATGGGG136950.021.552034
Produced by FastQC (version 0.11.5)