FastQCFastQC Report
Mon 6 Jun 2016
ERR523037_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523037_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12090997
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT627870.519287201874254No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT332080.2746506346829794No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT274000.22661489370975774No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162370.13429000106442837No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT154280.12759907226839937No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA130300.10776613376051619No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG176150.047.06482
GTACCTG202050.041.5525671
ACCTGGG211050.038.8147283
CCTGGGG204800.028.3553314
CATGGGG324700.022.6346824
TATAACG8950.022.0674842
GTACATG575650.022.0445841
TACATGG585150.021.4731522
ACATGGG589300.020.5801563
ATGGGGG189900.020.4762025
TGGGGGG178100.019.9735766
GAGTACT306650.019.12430212-13
CTGGGGG140650.019.0547835
GTACTTT341150.017.1780114-15
AGTACTT325450.017.09548212-13
TATCACG12100.016.7112772
ATAACGC13200.015.6751793
GTACACG29150.015.489991
TAACGCA14400.015.3473394
GTACCCG23400.015.0752291
Produced by FastQC (version 0.11.5)