FastQCFastQC Report
Mon 6 Jun 2016
ERR523037_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523037_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12090997
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT630800.5217104925259679No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT290220.2400298337680507No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT261010.21587136279994115No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153990.1273592243881956No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT146830.12143746293213041No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC128400.10619471661435363No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG174200.048.3998152
GTACCTG208650.040.739581
ACCTGGG211250.039.0853423
CCTGGGG206500.028.4237424
TATAACG9350.023.121482
CATGGGG312200.021.8410434
GTACATG563700.021.2430741
CTGGGGG128550.021.1296775
TACATGG574800.020.342442
TGGGGGG159650.020.3396956
ACATGGG574100.019.8173773
ATGGGGG179450.019.404925
GAGTACT310400.018.99919312-13
TATCACG12450.018.496782
TACCGTA9300.018.190937
GTACTTT343200.017.09194614-15
AGTACTT328450.017.01046212-13
GAGTACG22900.016.6297631
GTATATA44000.016.4552521
ATAACGC12600.016.0375043
Produced by FastQC (version 0.11.5)