FastQCFastQC Report
Mon 6 Jun 2016
ERR523036_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523036_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7754776
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT318300.4104567301492655No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT241990.3120528562011333No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT186230.24014878057083788No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104670.13497488515464534No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT94570.12195065337799571No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG260750.026.9579011
TACATGG266350.025.87752
ACATGGG268650.024.9053613
CATGGGG186600.023.305734
ATGGGGG110600.021.127085
GAGTACT213250.020.63297312-13
AGAGTAC326750.019.6047610-11
GTACTTT233050.018.62786514-15
GAGTACA204400.018.6095921
AGTACTT225250.018.14669212-13
AGTACAT199700.017.6572992
TACCTGG54800.017.3305322
GTATCAA597450.016.9123541
TATAACG6700.016.1396482
TATACTG34150.016.1076955
ATAACGC7300.016.102453
TCAACGC631450.015.8216814
CAACGCA634150.015.7248645
ATCAACG643850.015.5184753
AACGCAG645000.015.4717926
Produced by FastQC (version 0.11.5)