FastQCFastQC Report
Mon 6 Jun 2016
ERR523036_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523036_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7754776
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT330110.42568605463265474No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220060.2837735093831208No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180750.23308216768608148No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100990.13022942248750963No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT92470.11924264479077151No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG257150.026.2815361
TACATGG263400.025.2856462
ACATGGG269000.023.9344713
CATGGGG188450.021.8954524
GAGTACT209300.020.33817912-13
ATGGGGG109500.019.7421135
AGAGTAC323050.019.07028810-11
GTACTTT225250.018.6553114-15
TACCTGG54400.018.1443062
GAGTACA202400.017.5900021
AGTACTT219800.017.55934312-13
AGTACAT200750.016.763992
GTGTAGC44100.015.8901571
GTACCTG65950.015.7600641
GTATCAA606800.015.4856851
GTATAGC24600.015.1033231
ACTTTTT278850.015.10084716-17
GTACAGG54100.014.6916291
TACACCG9950.014.6416025
TACTTTT263000.014.56570914-15
Produced by FastQC (version 0.11.5)