FastQCFastQC Report
Mon 6 Jun 2016
ERR523035_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523035_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12653303
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT408840.3231093098774288No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT317060.2505748894182017No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT201460.1592153447996938No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA143940.11375685858467154No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG224650.056.620792
GTACCTG259400.049.5761531
ACCTGGG254100.049.2450073
CCTGGGG217500.036.362554
TATAACG13400.027.722822
GTATCAA537850.026.962281
GGTATCA382700.025.4791621
CTGGGGG140050.024.7755265
TAACGCA15950.024.1735654
CATGGGG310400.023.9344224
TCAACGC614450.023.3246944
GTACATG611550.023.2283971
CAACGCA620400.023.1368735
ATCAACG622650.022.9740313
GTACCCG22750.022.9501691
ATAACGC15800.022.9168873
AACGCAG636250.022.7262256
TATCAAC636300.022.5769042
TACATGG628400.022.2396372
ACATGGG622150.021.8889753
Produced by FastQC (version 0.11.5)