FastQCFastQC Report
Mon 6 Jun 2016
ERR523035_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523035_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12653303
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT349720.2763863316953684No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT296400.23424713689382134No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT189590.14983439501922935No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA141690.11197866675602411No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG225200.058.4751972
GTACCTG263900.050.669411
ACCTGGG256000.050.4074553
CCTGGGG225200.036.1629944
CTGGGGG133600.026.4510045
TAACGCA15000.026.3161414
GTATCAA524100.026.0518231
GGTATCA379000.024.7739071
TATAACG14650.024.70162
ATAACGC16100.023.3506153
GTACACG28300.022.594781
CATGGGG306500.022.3236474
TCAACGC605850.022.0984384
GTACATG624500.021.961381
CAACGCA611450.021.9266175
ATCAACG617500.021.6739083
AACGCAG626800.021.5396756
TATCACG13850.021.3777872
TATCAAC631050.021.292292
TACATGG640600.021.1509422
Produced by FastQC (version 0.11.5)