FastQCFastQC Report
Mon 6 Jun 2016
ERR523034_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523034_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8675861
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT196890.226940012063356No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162080.1868171931293044No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95620.11021384505814466No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA119700.050.15691
GTATCAA213350.040.8434521
TCAACGC254900.033.5896344
CAACGCA260350.032.9043545
ATCAACG260800.032.7956053
TATCAAC269000.032.0112652
AACGCAG268600.031.8517196
GTACATG279800.027.3262351
ACGCAGA312500.027.2266887
CGCAGAG324100.026.2213978
TACATGG285450.026.09932
ACATGGG290100.024.752983
GCAGAGT358900.023.2723149
CATGGGG206900.022.0084824
TACCTGG67550.021.5012042
GAGTACT207400.020.45480512-13
CAGAGTA352050.019.98233410-11
GTGGTAT67250.019.380351
AGAGTAC331250.019.21618310-11
ATGGGGG114550.018.9527025
Produced by FastQC (version 0.11.5)