FastQCFastQC Report
Mon 6 Jun 2016
ERR523034_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523034_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8675861
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT184890.21310853182179845No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158830.18307116723054923No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88820.10237600625459538No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA117950.050.6943051
GTATCAA205500.039.4672661
TCAACGC248650.031.9577264
CAACGCA254200.031.3154455
ATCAACG256500.030.9624353
AACGCAG267450.029.8516946
TATCAAC268700.029.8430392
ACGCAGA306600.026.0245847
GTACATG268250.025.8329851
CGCAGAG318750.024.9649188
TACATGG275900.024.2741832
ACATGGG280650.023.0403543
CATGGGG196150.021.7769134
GCAGAGT365500.021.5789579
TACCTGG70250.020.5531482
GTGGTAT65000.020.4102041
GAGTACT208400.019.577712-13
AGAGTAC328000.019.44663410-11
TGGTATC63950.018.9742872
CAGAGTA356100.018.9482710-11
Produced by FastQC (version 0.11.5)