FastQCFastQC Report
Mon 6 Jun 2016
ERR523033_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523033_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9392832
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT372470.3965470690841697No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT292250.3111415172761527No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT166630.17740123532497973No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT154500.1644871323153656No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG137950.046.335222
ACCTGGG148800.041.6602023
GTACCTG169500.038.3757321
CCTGGGG136650.029.0492384
TATAACG9850.028.6502572
TATCACG9200.027.6070142
GTACATG388950.027.2561421
TACATGG397150.026.078122
GTATCAA516900.025.4956341
CATGGGG241200.025.0179234
ACATGGG401050.024.8860363
ATGGGGG149500.024.3492455
TAACGCA12950.024.3336334
GGTATCA374350.023.9343361
TCAACGC583750.022.253524
GTACACG20800.022.1598591
CAACGCA590950.021.973615
ATCAACG592700.021.9098973
ATAACGC15050.021.8761253
TATCAAC603900.021.5505142
Produced by FastQC (version 0.11.5)