FastQCFastQC Report
Mon 6 Jun 2016
ERR523033_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523033_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9392832
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT352080.37483902618507386No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT301120.320584888561831No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT165700.17641111860618822No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT155550.16560500603013023No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG145300.049.4625552
ACCTGGG157550.043.7051963
GTACCTG178450.040.9626771
TATAACG10450.035.0843122
CCTGGGG139950.031.1329784
TAACGCA11750.029.2010754
GTACATG391550.025.4684491
GGTATCA389300.025.0719181
TACATGG398700.024.4274522
CATGGGG240400.024.126514
GTATCAA527450.023.9986211
ACATGGG398950.023.668753
ATAACGC15700.023.650553
TATCACG8600.023.5019912
ATGGGGG143800.023.1743815
GAGTACT329250.021.154512-13
TCAACGC595100.021.080094
CAACGCA601000.020.857845
ATCAACG603450.020.7884043
AACGCAG616400.020.4335776
Produced by FastQC (version 0.11.5)