FastQCFastQC Report
Mon 6 Jun 2016
ERR523032_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523032_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10505755
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT828110.7882441576069497No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT291410.277381301962591No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT256680.24432323045797277No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT214540.20421188196374274No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC179240.17061125069069286No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC177660.16910731308696997No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA133030.126625835078012No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124770.11876347773196691No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG362300.027.3571971
TACATGG369850.026.2639142
ACATGGG378450.025.0463873
CATGGGG263600.023.0969284
ATGGGGG151950.020.4518535
GAGTACT267400.019.26426112-13
AGAGTAC435450.018.32911510-11
GTACTTT285800.017.78715314-15
AGTACTT282350.016.94643612-13
GAGTACA284800.016.8395121
AGTACAT275650.016.3403852
CATGGGA130100.015.61124
TACCTGG69750.014.9645452
ACTTTTT349300.014.802423516-17
GTATAGG29450.014.688311
GTGTAGC51200.014.5096331
TATACCG7850.014.3738645
TACTTTT333400.014.21893114-15
GTATAAG35550.013.8873381
GTATAGC31150.013.7357591
Produced by FastQC (version 0.11.5)