FastQCFastQC Report
Mon 6 Jun 2016
ERR523032_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523032_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10505755
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT849200.8083188690389219No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT269460.25648799158175684No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT256240.24390441239111327No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT205750.19584503921898044No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC186880.17788345530616315No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC179250.17062076928312148No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG147510.14040875691466248No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA136080.12952900576874293No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA124850.11883962647139591No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116920.11129138267549547No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG354950.026.5399761
TACATGG362800.025.6268712
ACATGGG369400.024.3246693
CATGGGG254800.023.2026254
ATGGGGG142950.019.6920225
GAGTACT268300.019.60751212-13
AGAGTAC427150.018.6716810-11
GTACTTT286350.018.1549714-15
GAGTACA280350.017.3462961
AGTACTT283050.017.25700612-13
AGTACAT273700.016.2462432
ATGGGGA109000.015.2624565
TACCTGG68100.014.8397962
ACTTTTT352000.014.80634616-17
TGGGGGG133350.014.3080586
TACTTTT335750.014.30078714-15
CATGGGA133550.014.0405764
GTGTAGC54650.013.6833921
TCTATAC31450.013.4486243
GTATATA48100.013.2978261
Produced by FastQC (version 0.11.5)