FastQCFastQC Report
Mon 6 Jun 2016
ERR523031_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523031_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8225020
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT323110.39283795054601695No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT262390.31901442184943013No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162930.19809070373081158No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127660.15520934903501754No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT104660.12724589119540136No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG130900.051.762412
GTACCTG150600.045.5346371
ACCTGGG157600.042.129073
CCTGGGG139100.031.5370314
GGTATCA335350.023.4923171
GTACATG346400.022.8515451
GTATCAA443900.022.7168941
TACATGG351650.022.0763152
CATGGGG199600.021.6246174
CTGGGGG87700.021.604425
ACATGGG357000.021.100743
TATAACG7200.020.8981382
GTACCCG13850.020.375621
GAGTACT273450.020.0789812-13
TCAACGC502500.019.742984
CAACGCA505900.019.6182695
ATGGGGG121300.019.4571885
TATCAAC512900.019.444562
ATCAACG509750.019.4269473
AACGCAG520200.019.1779566
Produced by FastQC (version 0.11.5)