FastQCFastQC Report
Mon 6 Jun 2016
ERR523031_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523031_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8225020
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT285020.34652803275858296No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT251700.3060174929665824No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159780.19426092580929893No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113180.137604528621207No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT104120.12658935783742775No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG138000.052.4489482
GTACCTG158250.046.5045621
ACCTGGG157750.045.0717053
CCTGGGG143000.032.928064
TATCACG7850.023.3501722
CTGGGGG89000.023.337885
TATAACG8500.023.2233872
GTATCAA444550.023.1870161
GGTATCA333650.023.1141361
GTACATG351650.022.372281
TACATGG361850.021.3404792
CATGGGG199650.020.901534
ACATGGG358500.020.7897113
TCAACGC503400.020.2852784
CAACGCA508350.020.1429735
ATCAACG510250.020.0221613
GAGTACT277250.019.87990812-13
AACGCAG521550.019.8405256
TATCAAC517550.019.815162
GTACCGG13200.019.5935761
Produced by FastQC (version 0.11.5)