FastQCFastQC Report
Mon 6 Jun 2016
ERR523030_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523030_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8546648
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT192950.22576102350301544No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT157440.18421257082308762No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA140250.043.5472341
GTATCAA234600.037.1451151
TCAACGC280350.030.6565554
CAACGCA286200.029.9789125
ATCAACG288500.029.7773763
TATCAAC297900.028.9531292
AACGCAG297150.028.8839046
GTACATG297700.026.0791761
ACGCAGA339050.025.2177187
TACATGG310100.024.5854972
CGCAGAG350800.024.2647828
ACATGGG319050.023.3025673
CATGGGG222500.021.4150034
GCAGAGT392650.021.2589479
GAGTACT189300.020.18866512-13
AGAGTAC330950.019.13933610-11
CAGAGTA375950.018.88497410-11
GTACTTT202050.018.77700814-15
TACCTGG65650.018.6152342
ATGGGGG120500.018.17125
Produced by FastQC (version 0.11.5)