FastQCFastQC Report
Mon 6 Jun 2016
ERR523030_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523030_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8546648
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175250.20505114987770645No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151240.17695826480744264No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA151200.039.480761
GTATCAA245250.035.7146451
TCAACGC290150.029.57414
CAACGCA297100.028.8822825
ATCAACG299950.028.6083553
TATCAAC308650.028.050372
AACGCAG308950.027.8804656
GTACATG298250.025.4565031
ACGCAGA352800.024.348717
TACATGG309650.024.0870252
CGCAGAG365550.023.4801948
ACATGGG313400.022.9870993
CATGGGG222050.021.0997714
GCAGAGT409450.020.8019969
GAGTACT191200.019.69280412-13
AGAGTAC335750.019.4673910-11
GTACTTT203600.018.1906914-15
CAGAGTA394400.017.89539310-11
ATGGGGG122250.017.4518055
GAGTACA239550.017.0647241
Produced by FastQC (version 0.11.5)