FastQCFastQC Report
Mon 6 Jun 2016
ERR523029_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523029_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10243293
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT284470.2777134267271277No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT237820.23217143158943124No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT208080.20313779953380226No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131570.12844502251375606No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG169100.053.812052
GTACCTG199050.046.2967641
ACCTGGG198350.044.9519233
CCTGGGG177650.033.428964
TATAACG10400.023.9654032
GTACATG450700.022.7638911
CATGGGG257550.022.108914
CTGGGGG111050.022.1009585
TACATGG460050.021.94672
GTACCCG18650.021.4396881
ACATGGG465750.021.1227763
ATGGGGG150250.021.091365
TGGGGGG152950.020.739626
GAGTACT265200.019.71218312-13
ATAACGC13200.019.5943553
GTACACG21650.018.903391
TAACGCA14300.018.7423294
GTATCAA631650.018.0076981
TATCACG10450.018.0005552
TACCCGG21450.017.5390012
Produced by FastQC (version 0.11.5)