FastQCFastQC Report
Mon 6 Jun 2016
ERR523029_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523029_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10243293
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT257600.25148162802723695No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT227540.22213559643368594No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT220320.21508708185932007No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130950.12783974840903214No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG180250.053.1921962
GTACCTG203950.047.5416561
ACCTGGG203750.046.4974063
CCTGGGG181000.034.712724
TATAACG10600.029.2638262
CTGGGGG110700.025.003535
TAACGCA14150.023.247564
TGGGGGG146750.022.960156
CATGGGG254800.021.9289684
GTACATG449250.021.5619741
TATCACG9600.021.5414282
ATAACGC14850.021.5188123
TACATGG457700.021.0198672
ATGGGGG150750.020.4493835
ACATGGG462200.020.1210963
GAGTACT281800.019.56805212-13
GTACCCG18500.019.0633351
GTATAAC30550.018.4705311
GTATCAA639400.017.4589231
AGTACTT301700.017.4437312-13
Produced by FastQC (version 0.11.5)