##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR523028_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 892069 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.371307600645242 29.0 27.0 30.0 23.0 30.0 2 31.80568991860495 34.0 34.0 34.0 26.0 35.0 3 30.838951919638504 34.0 33.0 34.0 23.0 34.0 4 31.300111314259322 34.0 32.0 35.0 22.0 35.0 5 31.55766426139682 35.0 32.0 35.0 23.0 36.0 6 31.944564826263438 35.0 34.0 36.0 22.0 36.0 7 32.099177305791365 36.0 33.0 36.0 24.0 36.0 8 32.52963952340009 35.0 34.0 36.0 23.0 38.0 9 32.63893824356636 36.0 33.0 37.0 23.0 38.0 10-11 32.72052498181195 36.0 33.0 37.0 21.5 37.0 12-13 32.89116817196876 36.0 34.0 36.5 22.5 38.5 14-15 32.89016376535896 36.0 33.5 37.0 21.0 38.0 16-17 32.95042423848379 36.5 34.0 37.0 22.5 38.0 18-19 33.045850152846924 36.0 34.0 37.5 22.0 37.5 20-21 33.36615553281192 37.0 34.5 37.5 23.5 38.0 22-23 33.360138621564026 37.0 34.0 38.0 23.5 38.0 24-25 33.2093005137495 36.5 33.5 37.0 22.0 38.0 26-27 33.32221386462258 37.0 34.5 37.5 22.5 38.0 28-29 33.26759701323552 37.0 33.0 37.5 20.5 38.0 30-31 33.29063951331119 37.0 33.5 38.0 21.5 38.5 32-33 33.14048520910378 37.5 33.0 37.5 20.5 38.5 34-35 32.75001036915306 36.5 33.5 37.5 16.0 39.0 36-37 32.33826643454711 36.5 33.0 37.0 12.0 37.5 38-39 32.24116688283081 36.5 33.0 37.5 11.5 38.0 40-41 32.00061486275165 37.0 32.0 37.5 11.0 39.0 42-43 31.131063852684044 36.0 30.5 37.5 10.5 38.0 44-45 30.59250293419007 36.0 26.5 37.0 10.5 38.0 46-47 29.585321314831027 36.0 22.0 37.0 10.0 38.0 48-49 28.901408411232765 35.5 21.0 37.5 8.0 38.0 50-51 28.414376017998606 35.5 20.5 37.0 6.5 38.5 52-53 28.073604732369354 36.0 20.0 37.0 7.0 38.5 54-55 28.01754516747023 34.5 12.0 38.0 7.0 38.5 56-57 27.78155557473693 35.5 11.5 37.5 7.0 38.0 58-59 27.661242011548435 35.5 12.0 37.0 7.0 38.5 60-61 27.276308222794427 35.5 12.0 37.0 7.0 38.0 62-63 27.069921721301824 35.0 12.0 36.5 7.0 37.5 64-65 26.872161234164622 34.0 11.5 36.5 7.0 38.0 66-67 26.982226711162475 34.0 11.0 37.0 7.0 38.0 68-69 26.704919126211088 34.5 11.0 37.5 7.0 38.0 70-71 26.142757454860554 33.5 10.5 36.5 7.0 38.0 72-73 26.05037054308579 34.0 11.0 37.0 7.0 38.0 74-75 25.626306373161718 33.0 11.0 37.0 6.5 38.0 76-77 25.252504010340004 32.0 11.0 37.0 6.5 37.5 78-79 25.11002343989086 31.5 10.5 37.0 7.0 37.5 80-81 24.913190571581346 31.5 10.5 37.0 6.5 37.0 82-83 24.884359842119835 31.5 10.0 37.0 6.0 38.0 84-85 24.440641923438655 30.5 10.5 37.0 6.0 37.0 86-87 24.33922880404991 31.0 10.5 37.0 6.5 38.0 88-89 24.11004866215506 29.5 10.5 37.0 6.5 37.5 90-91 23.8555700287758 29.5 10.5 36.5 6.0 38.0 92-93 23.72641129778078 27.5 10.5 37.0 6.5 37.0 94-95 23.528872766568504 26.5 10.5 36.5 6.0 38.0 96-97 23.48571803302211 27.0 10.5 36.0 6.0 37.0 98-99 23.55791816552307 24.0 11.5 36.0 7.0 37.5 100 23.18275492142424 21.0 11.0 35.0 7.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2500473597050572 1101 2 0.36200265241144436 1101 3 0.4770462431646578 1101 4 0.2599664874313987 1101 5 0.5643931304238912 1101 6 0.19558590292371036 1101 7 0.5670394452053173 1101 8 0.28928158786815317 1101 9 0.24266062731511084 1101 10-11 0.2689779303506299 1101 12-13 0.07708722796818535 1101 14-15 0.29383217923021476 1101 16-17 0.3341664171644396 1101 18-19 0.22519370702962505 1101 20-21 0.030162011449483828 1101 22-23 0.16835514306637833 1101 24-25 -0.047311075423230875 1101 26-27 0.17358944517339836 1101 28-29 0.23637853215775095 1101 30-31 0.1171203648859489 1101 32-33 0.0770643970747642 1101 34-35 -0.6616123147823743 1101 36-37 -0.02092456503495299 1101 38-39 -0.049426682477651696 1101 40-41 0.2906740749774279 1101 42-43 -0.17188442062131415 1101 44-45 0.05914934015174467 1101 46-47 0.382987262225015 1101 48-49 0.6730312431312875 1101 50-51 0.7377783659500814 1101 52-53 0.8344958745724185 1101 54-55 0.7346069588012369 1101 56-57 0.4368694324534097 1101 58-59 0.4322649850539655 1101 60-61 0.9613623232599444 1101 62-63 0.5646380199800767 1101 64-65 0.8277816724880118 1101 66-67 0.6230578474373871 1101 68-69 0.46975060163360993 1101 70-71 0.57692800132941 1101 72-73 0.5706279648603036 1101 74-75 0.8131264349016547 1101 76-77 0.19024570537997576 1101 78-79 0.41711303284544243 1101 80-81 0.46846946477127815 1101 82-83 -0.06436534092100388 1101 84-85 -0.558615380747284 1101 86-87 -0.2988483802575743 1101 88-89 -0.396343324183114 1101 90-91 0.0027464701057589025 1101 92-93 -0.3980643059376803 1101 94-95 -0.586404940117685 1101 96-97 -0.6073839769332814 1101 98-99 -0.7515419251794953 1101 100 -1.5308448395865746 1102 1 -0.026415805274332627 1102 2 0.24110750672592118 1102 3 -0.05112336338628154 1102 4 0.007247678715504691 1102 5 -0.051147125892885725 1102 6 0.036813923900183454 1102 7 0.30826045532899116 1102 8 -0.053711765843395654 1102 9 -1.5477176783019857 1102 10-11 -0.004392046373340008 1102 12-13 -0.1637005114524399 1102 14-15 -0.3744633422370143 1102 16-17 -0.15966893996839815 1102 18-19 0.03197951145487821 1102 20-21 -0.03955320770977977 1102 22-23 -0.06540631967795463 1102 24-25 -0.06703190326472708 1102 26-27 0.21206149991413525 1102 28-29 -0.21332280551296634 1102 30-31 0.0988816313263925 1102 32-33 0.12353723703102304 1102 34-35 0.05368957253919859 1102 36-37 -0.007059201452129571 1102 38-39 0.004230879428170908 1102 40-41 -0.19544583864886889 1102 42-43 -0.021535280151869785 1102 44-45 -0.11021673853806746 1102 46-47 -0.38908851107204967 1102 48-49 -0.06133735946552932 1102 50-51 -0.16112934318366 1102 52-53 -0.07005486998015087 1102 54-55 -0.07743908847995229 1102 56-57 -0.3715828516824331 1102 58-59 -0.34589059979137815 1102 60-61 0.1563846177718453 1102 62-63 -0.19228379498949622 1102 64-65 0.25006286377212206 1102 66-67 0.18391835951007707 1102 68-69 -0.013242471643820863 1102 70-71 -0.06174877031309478 1102 72-73 -0.003414352647194363 1102 74-75 -0.12935933306715697 1102 76-77 -0.25988890299378653 1102 78-79 -0.3141799086279562 1102 80-81 -0.41887664700976046 1102 82-83 -0.3391725424689973 1102 84-85 -0.3317848470811704 1102 86-87 -0.5095153927929807 1102 88-89 -0.7545551360679106 1102 90-91 -0.5254280440421439 1102 92-93 -0.872337981587588 1102 94-95 -0.8543134625103477 1102 96-97 -0.5184246679229325 1102 98-99 -1.532906377351459 1102 100 -0.6345087112599259 1103 1 0.26352777209888956 1103 2 0.3066498443132879 1103 3 0.1597914253896704 1103 4 0.3118762583909884 1103 5 0.27259779710156806 1103 6 0.25626014372163297 1103 7 0.41679532258805807 1103 8 0.2183181429594967 1103 9 0.06753361199621111 1103 10-11 0.3358075289106637 1103 12-13 0.4186483466937929 1103 14-15 0.31175881584255904 1103 16-17 0.3290039256275392 1103 18-19 0.16128903870062317 1103 20-21 0.05750333174826494 1103 22-23 0.5022884620881243 1103 24-25 0.4471259838762691 1103 26-27 0.4211516592857194 1103 28-29 0.038980488441360706 1103 30-31 0.1130560122925175 1103 32-33 0.33151543769213987 1103 34-35 0.21970198414260267 1103 36-37 0.2806735161117331 1103 38-39 0.26508072376770286 1103 40-41 0.16859212463862505 1103 42-43 0.2581566976639351 1103 44-45 0.10160727234785583 1103 46-47 0.2646731119203416 1103 48-49 0.46721826252348464 1103 50-51 0.017326284990542007 1103 52-53 -0.09102264516931413 1103 54-55 -0.4640474769645131 1103 56-57 -0.35745932667710534 1103 58-59 -0.5249997957861261 1103 60-61 -0.46431100930276514 1103 62-63 -0.3327857570674446 1103 64-65 -0.29989624528642267 1103 66-67 -0.24365381815590936 1103 68-69 -0.1983045640229868 1103 70-71 -0.6223656393727417 1103 72-73 -0.3969886332758854 1103 74-75 -0.27027432801592255 1103 76-77 -0.9911522612740313 1103 78-79 -1.053877796731097 1103 80-81 -0.42900660460688655 1103 82-83 -0.5011893249360604 1103 84-85 -0.8078365002435177 1103 86-87 -0.7433682816177267 1103 88-89 -0.5324788383818557 1103 90-91 -0.19380044712373845 1103 92-93 -1.0942795797017943 1103 94-95 -1.2681772097699664 1103 96-97 -1.3246888852565704 1103 98-99 -1.238534207932382 1103 100 -0.27780362772557154 1104 1 0.2528556444298111 1104 2 0.2224314712229578 1104 3 0.13406524608117465 1104 4 0.33516927634864047 1104 5 0.6588380812077084 1104 6 0.3241290126575791 1104 7 0.22090838432307436 1104 8 0.21230154776607435 1104 9 0.12419430394653119 1104 10-11 0.4678620216739944 1104 12-13 0.28993299446472776 1104 14-15 0.4038280778813217 1104 16-17 0.4122800245286413 1104 18-19 0.06977536944304319 1104 20-21 0.3191191295683282 1104 22-23 0.34244316257056795 1104 24-25 0.31983939851041754 1104 26-27 0.39161031648217914 1104 28-29 0.23814723886572864 1104 30-31 0.5485395455275821 1104 32-33 -0.4382416091228265 1104 34-35 0.308145933485946 1104 36-37 0.20912977783780917 1104 38-39 0.3164535125642729 1104 40-41 -0.05216736231750474 1104 42-43 0.02518254529191921 1104 44-45 -0.06119676812386743 1104 46-47 -0.1845027122554832 1104 48-49 -0.29692352810458544 1104 50-51 -0.8897166212901695 1104 52-53 -0.7266284646547057 1104 54-55 -0.636009724574631 1104 56-57 -0.5785077777179346 1104 58-59 -0.6410551707972907 1104 60-61 -0.4575391987962014 1104 62-63 -0.5072523600782546 1104 64-65 -0.2652847693639728 1104 66-67 -0.48887195114081194 1104 68-69 -0.3114395870284348 1104 70-71 -0.3507373058160539 1104 72-73 -0.5341928289785578 1104 74-75 -0.4443186525937861 1104 76-77 -0.5226570242955617 1104 78-79 -0.5620811660244058 1104 80-81 -0.4117113363588736 1104 82-83 -0.6256093028240279 1104 84-85 -0.35977116364921713 1104 86-87 -0.46451724190540844 1104 88-89 -0.292055136067912 1104 90-91 -0.6821218310243928 1104 92-93 -0.6749452301082961 1104 94-95 -0.5129765542263272 1104 96-97 -0.41513324780459016 1104 98-99 -0.7358742027952445 1104 100 -0.4321048651060764 1105 1 0.07935091822381324 1105 2 0.012384209162988213 1105 3 8.473516874296649E-4 1105 4 0.057205890864068465 1105 5 -0.0383098979317964 1105 6 0.14625797832467669 1105 7 0.1530302878432792 1105 8 -0.12202320234257513 1105 9 0.15039351081311736 1105 10-11 0.07417245735413758 1105 12-13 0.015402219801536887 1105 14-15 0.011800155238994137 1105 16-17 -0.22733182901329485 1105 18-19 -0.035151836119752033 1105 20-21 -0.1831529803838663 1105 22-23 0.029749632583602192 1105 24-25 -0.2814670986647272 1105 26-27 0.08417061635180545 1105 28-29 -0.1589919125901531 1105 30-31 0.05058824717903576 1105 32-33 -0.0019734105135285063 1105 34-35 -0.10665877099037857 1105 36-37 -0.04989007765979636 1105 38-39 0.08273855167769995 1105 40-41 0.2459007330758034 1105 42-43 0.17483419131538724 1105 44-45 0.7284286664697888 1105 46-47 0.23941080360718914 1105 48-49 -0.1113758042888513 1105 50-51 0.07658335698014795 1105 52-53 0.12019017398067078 1105 54-55 -0.11492446273195256 1105 56-57 -0.06124286572315185 1105 58-59 -0.05553189287465088 1105 60-61 -0.21598525485878284 1105 62-63 -0.13373065755761004 1105 64-65 0.03617926801107885 1105 66-67 0.07052953191003652 1105 68-69 -0.36335112005755477 1105 70-71 0.07358703686104562 1105 72-73 -0.051062532368387537 1105 74-75 -0.029155408352153245 1105 76-77 0.0168971097765791 1105 78-79 -0.23050725794096394 1105 80-81 -0.4220458376626546 1105 82-83 -0.3346427393733542 1105 84-85 -0.12749239898167986 1105 86-87 -0.26501634555599196 1105 88-89 -0.042951485481211193 1105 90-91 -0.7120626176389067 1105 92-93 -0.03728633180321239 1105 94-95 -0.7391628590937742 1105 96-97 -0.3531520439801703 1105 98-99 -0.5197582477756839 1105 100 0.09352257948323128 1106 1 -0.011437423153822124 1106 2 0.030107859404317594 1106 3 -0.07351191048191197 1106 4 0.018312636851273822 1106 5 -0.0031057972096810715 1106 6 -0.20956197902182438 1106 7 -0.03056682369187058 1106 8 -0.5257535125158057 1106 9 -0.5707162284019134 1106 10-11 -0.09951332855524697 1106 12-13 -0.2947335979108914 1106 14-15 -0.3275832398296572 1106 16-17 -0.22216289871809636 1106 18-19 -0.38020552714380784 1106 20-21 -0.24343658872000873 1106 22-23 -0.4610742533744201 1106 24-25 -0.4128454800767756 1106 26-27 -0.8484293761651216 1106 28-29 -0.36610831173708647 1106 30-31 -0.31502891898252017 1106 32-33 -0.10702822399802159 1106 34-35 -0.08361465829534609 1106 36-37 -0.1683788717631387 1106 38-39 -0.26949327381711896 1106 40-41 -0.10758450656949847 1106 42-43 0.3952143312487877 1106 44-45 0.07726317838658048 1106 46-47 -0.019937421034558866 1106 48-49 0.30098809738361254 1106 50-51 0.4262166865719479 1106 52-53 0.4913242331717491 1106 54-55 0.6594497655765004 1106 56-57 0.24281295797572966 1106 58-59 0.7081247090634655 1106 60-61 0.5267243646271318 1106 62-63 0.7626138629647059 1106 64-65 0.5720549742257681 1106 66-67 0.39787266806448685 1106 68-69 0.21312023849372252 1106 70-71 0.3939522106553319 1106 72-73 0.3680043288580883 1106 74-75 0.44686883679320744 1106 76-77 0.40100494080887117 1106 78-79 -0.043082556328364774 1106 80-81 0.4032787404792977 1106 82-83 0.13745663579033618 1106 84-85 0.18051448544230908 1106 86-87 0.0335525173915876 1106 88-89 0.387081814178309 1106 90-91 0.15720254493077945 1106 92-93 0.13262237474821603 1106 94-95 0.10208569106149668 1106 96-97 -0.0625956777587433 1106 98-99 0.05840989679721531 1106 100 0.17781935802178594 1107 1 0.16358989564371385 1107 2 0.002946740837067807 1107 3 0.05523479842804946 1107 4 0.20094521552574207 1107 5 0.29555649844937903 1107 6 0.30347403778748827 1107 7 0.15128469405984646 1107 8 -0.13478668376983194 1107 9 -0.0267861351879759 1107 10-11 0.139564652860642 1107 12-13 -0.07847506307850693 1107 14-15 -0.004477037545882467 1107 16-17 -0.0015608584256199265 1107 18-19 -0.06294994787528907 1107 20-21 -0.12227680492353699 1107 22-23 -0.09872982384224827 1107 24-25 -0.223038508466324 1107 26-27 -0.058941796133886726 1107 28-29 0.013388288997774112 1107 30-31 -0.057067964694752504 1107 32-33 -0.2524999041224021 1107 34-35 -0.03771907844011935 1107 36-37 -0.36117074286242 1107 38-39 0.11624081344168502 1107 40-41 -0.08973378087955908 1107 42-43 0.3925191210223886 1107 44-45 0.12642968004037414 1107 46-47 0.38538186053606793 1107 48-49 0.707974008951382 1107 50-51 0.5594352442861172 1107 52-53 0.6657501717282948 1107 54-55 0.6265718677671366 1107 56-57 0.608089116182736 1107 58-59 0.4605632326016327 1107 60-61 0.48720561549803065 1107 62-63 0.5559590772247525 1107 64-65 0.48373825218811817 1107 66-67 0.7711207814173271 1107 68-69 0.6999613787693306 1107 70-71 0.6613904041677614 1107 72-73 0.6150579323724941 1107 74-75 0.8334719918392999 1107 76-77 0.7401228920090617 1107 78-79 0.683879813403145 1107 80-81 0.8387458735823508 1107 82-83 0.6416002923288779 1107 84-85 0.49967504663274553 1107 86-87 0.39239867405847306 1107 88-89 0.09241649695023568 1107 90-91 -0.19619085308760376 1107 92-93 0.3650430035572647 1107 94-95 0.4478371596760482 1107 96-97 0.40487111798465847 1107 98-99 0.5170906738978331 1107 100 0.3670927880159951 1108 1 0.24601444266716754 1108 2 0.12885260153869638 1108 3 0.29831157747502957 1108 4 0.14082673763040532 1108 5 0.25045162617219674 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0.5732996514167397 2316 88-89 0.3186216352397011 2316 90-91 0.6314190867805429 2316 92-93 0.66207125483459 2316 94-95 0.676791872764646 2316 96-97 1.1971885231036268 2316 98-99 1.01886047549376 2316 100 1.2553227449266728 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 50.0 1 28.0 2 22.0 3 23.0 4 23.0 5 23.0 6 43.0 7 86.0 8 116.0 9 140.0 10 146.0 11 181.0 12 254.0 13 964.0 14 3416.0 15 8501.0 16 14978.0 17 23360.0 18 32437.0 19 38583.0 20 44289.0 21 52466.0 22 53890.0 23 40366.0 24 26742.0 25 21279.0 26 18028.0 27 16878.0 28 17504.0 29 17491.0 30 18882.0 31 22248.0 32 28403.0 33 37097.0 34 53581.0 35 88583.0 36 165749.0 37 40579.0 38 4640.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 53.35805207080158 16.670460062271488 14.804670025036412 15.166817841890524 2 17.296605530929778 23.311177610681405 36.230663280871454 23.161553577517363 3 25.402862958150724 29.847875895095182 30.571918415342115 14.17734273141198 4 12.848964511817234 18.77828892213385 35.5812212621587 32.791525303890225 5 9.937534790181537 38.625518485931295 32.45273024366595 18.98421648022122 6 24.586480419728684 33.47004205588081 16.650606383205467 25.292871141185035 7 19.349139507283507 38.94999921477656 21.41932502193017 20.281536256009762 8 26.767792904711534 25.6819739470753 20.99982837778872 26.550404770424446 9 27.62383034474404 17.63832274072296 21.992771749250142 32.74507516528286 10-11 24.31752019628864 26.048222143475513 22.95250451921395 26.6817531410219 12-13 30.77553684456068 26.035615436720306 25.15487410451142 18.033973614207596 14-15 27.181875123716086 25.32011975436784 30.632579625495637 16.865425496420436 16-17 17.325280975435223 30.228644268639492 31.294783483057913 21.151291272867372 18-19 17.38794188821248 28.393345353496553 35.648345732047574 18.5703670262434 20-21 21.03115494019364 24.754022235582315 34.39050680616997 19.824316018054073 22-23 18.742548282642026 26.156130793552517 34.43868309156962 20.662637832235838 24-25 20.3686402325704 27.007259973912095 32.89907705352506 19.72502273999244 26-27 19.332070422424632 24.416128022826612 36.68083235474562 19.57096920000314 28-29 17.71176926467471 28.386452060801755 35.94825816856751 17.953520505956018 30-31 19.153938851533027 26.857302112545344 34.59391835020375 19.394840685717874 32-33 16.613408498467297 27.911560227895244 36.87070740939897 18.604323864238488 34-35 17.03465344314063 27.32897558043564 35.6175090608964 20.018861915527324 36-37 17.40857502799081 27.139173624890038 36.63552963325729 18.81672171386186 38-39 18.069835111542194 26.939555849587638 35.7499593525563 19.240649686313866 40-41 16.479933522926068 28.722402145926594 34.784117720939825 20.013546610207506 42-43 16.91028795413642 28.867986856059947 33.31088087841631 20.91084431138732 44-45 17.88841370220284 26.9303532117167 33.853807647545466 21.32742543853499 46-47 18.253731853998417 28.769002851304176 33.72863415069224 19.248631144005167 48-49 18.1834909838774 28.03724371384701 32.92165232655401 20.857612975721572 50-51 16.03536500948579 28.523122110367282 33.66755185772321 21.77396102242372 52-53 17.59569314922197 29.099792801797587 29.80776673543358 23.49674731354686 54-55 17.35902856024359 26.67438024501361 30.500733874255957 25.46585732048684 56-57 19.64998895280436 24.865498991172338 29.997117635249637 25.48739442077366 58-59 20.506283549737432 24.294299109751023 30.59253868417187 24.606878656339674 60-61 18.1445972666913 24.79465425500603 32.636728506323095 24.424019971979575 62-63 16.283338350939804 24.67232787227136 33.158305764529054 25.886028012259775 64-65 15.916978297068338 26.156097396881638 32.089390736868076 25.837533569181947 66-67 17.630985757541772 24.162610743523608 30.545699226197154 27.66070427273747 68-69 17.182121527989995 23.682644954332662 31.94801510114436 27.187218416532982 70-71 18.413655553007395 23.61467090496299 31.815931291601927 26.15574225042769 72-73 17.857184908745218 21.82932319908406 34.33872173221203 25.974770159958688 74-75 17.28447611116252 23.93207545053571 32.253561804778606 26.529886633523166 76-77 17.88134096318996 21.749296967216104 32.83015905503422 27.539203014559714 78-79 18.687244141104603 21.433617125769047 33.68798978302381 26.191148950102537 80-81 19.629626099957452 21.072990028696857 32.83062911651911 26.46675475482658 82-83 18.699381605583095 20.770450714284515 33.404902868181814 27.12526481195058 84-85 18.948372767299052 21.811208043833872 31.97296148839629 27.267457700470786 86-87 19.691491115065205 21.548755862464862 30.879983585658977 27.879769436810953 88-89 20.07481709166355 21.02799782713137 30.123549669052373 28.773635412152704 90-91 21.434277958210902 19.812678459200157 30.103141218307638 28.649902364281303 92-93 22.017082649498416 20.398139109264253 30.377241375830067 27.207536865407274 94-95 20.934939748227656 19.768311582290263 30.901208623864324 28.395540045617757 96-97 20.495676955518142 19.427713123205507 31.709633596679936 28.366976324596415 98-99 21.83820915673243 19.916492950623528 30.001816589498993 28.243481303145057 100 21.522342970114057 17.8494814170228 31.138415510054866 29.489760102808276 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 9837.0 1 5546.5 2 1259.5 3 1119.0 4 789.5 5 771.5 6 1340.5 7 2414.0 8 3174.5 9 2832.0 10 1754.5 11 1017.0 12 883.0 13 854.5 14 922.5 15 1030.0 16 1159.0 17 1358.5 18 1629.5 19 1990.5 20 2463.5 21 3138.0 22 4160.5 23 5570.5 24 7430.0 25 9735.5 26 12406.0 27 15199.5 28 18029.0 29 20635.5 30 22336.0 31 22963.0 32 22379.0 33 20794.5 34 18670.0 35 16013.5 36 13356.0 37 10967.0 38 9046.0 39 7890.5 40 7281.0 41 6871.0 42 6761.0 43 7138.5 44 8180.0 45 9938.0 46 16162.5 47 35317.0 48 69954.0 49 85549.5 50 79640.5 51 65438.0 52 40899.0 53 23374.0 54 14255.0 55 10709.5 56 9472.0 57 8910.5 58 8364.0 59 8173.0 60 8129.0 61 7510.5 62 6803.5 63 6168.0 64 5643.0 65 5156.0 66 4566.5 67 4037.0 68 3669.0 69 3306.0 70 2843.0 71 2396.5 72 2009.5 73 1684.5 74 1393.0 75 1122.5 76 873.5 77 664.5 78 520.5 79 377.5 80 272.0 81 205.0 82 138.0 83 87.0 84 63.0 85 40.0 86 21.0 87 11.5 88 10.5 89 6.5 90 2.5 91 0.5 92 0.5 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.018832623933798844 2 0.05616157494543584 3 0.09225743748521695 4 0.1165829100663738 5 0.11355623836272755 6 0.07129493346366705 7 0.06759566804809941 8 0.06456899634445318 9 0.06356010577657109 10-11 0.03598376358779422 12-13 0.0422613048990605 14-15 0.04601661979062158 16-17 0.054648239093612716 18-19 0.057338613941298265 20-21 0.05885194979312139 22-23 0.05560108018550135 24-25 0.052462309529868204 26-27 0.05358329904973718 28-29 0.05044452839410404 30-31 0.04702551035850366 32-33 0.038505990007499415 34-35 0.03643215939574181 36-37 0.0289775790886131 38-39 0.02847313380467206 40-41 0.037328951011636996 42-43 0.06227096782872177 44-45 0.06798801438005356 46-47 0.08099149281053372 48-49 0.09018360687345933 50-51 0.08250482866235684 52-53 0.0730885166954574 54-55 0.06361615525256455 56-57 0.049323538874235066 58-59 0.03318128978812177 60-61 0.024605719961124085 62-63 0.023484730441255104 64-65 0.02847313380467206 66-67 0.041364513283165316 68-69 0.03906648476743391 70-71 0.040187474287302884 72-73 0.034470427735971094 74-75 0.055096634901560304 76-77 0.044447234462805005 78-79 0.024661769437117533 80-81 0.017879782841910212 82-83 0.018216079697870907 84-85 0.01748743650995607 86-87 0.018608426029825046 88-89 0.01799188179389711 90-91 0.02617510528894065 92-93 0.038730187911473216 94-95 0.034862774067925234 96-97 0.031219558128351057 98-99 0.03228449817222659 100 0.03497487301991214 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 892069.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.57878547552798 #Duplication Level Percentage of deduplicated Percentage of total 1 88.89852081936263 37.851910470594 2 5.071679729245361 4.318919263842441 3 1.8031365694233734 2.3032609551767185 4 0.9592415238045459 1.6337335624516929 5 0.6064678514761079 1.2911332272902782 6 0.43378798451576445 1.1082099320735033 7 0.3048306752674297 0.9085523950000556 8 0.24266439327037165 0.8265884114886648 9 0.1989159752384995 0.7622640573601955 >10 1.2358552504994968 10.025630303610551 >50 0.12428900994701536 3.6858796273816803 >100 0.09459624937214338 7.8666161768475735 >500 0.011824531171513497 3.727051500947129 >1k 0.011036229093412597 11.263948034022368 >5k 0.0021021388749357323 6.005216206227071 >10k+ 0.0010510694374678661 6.421085875686138 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 17586 1.9713721696415858 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14540 1.6299187618894952 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13674 1.5328410694688415 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 11591 1.299338952480133 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 7872 0.88244295004086 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 7507 0.8415268325656424 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 7461 0.836370280774245 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7238 0.8113722144811668 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7098 0.7956783612030012 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 6103 0.6841399039760377 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 5237 0.5870622115553842 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 5158 0.5782063943484193 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 4783 0.5361692873533325 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 4726 0.5297796470900794 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 4706 0.5275376680503414 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 4606 0.5163277728516517 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 4057 0.45478544821084466 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 3911 0.43841900122075755 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 3835 0.4298994808697534 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 3435 0.3850599000749942 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 3283 0.36802085937298573 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 3247 0.36398529710145744 No Hit GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA 3225 0.3615191201577456 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 3126 0.35042132391104275 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 3078 0.34504057421567164 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 3073 0.34448007945573716 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3052 0.3421260014640123 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 2734 0.3064785347321788 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 2654 0.29751061857322697 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 2634 0.29526863953348903 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 2554 0.2863007233745372 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 2317 0.2597332717536424 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 2229 0.24986856397879537 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 2001 0.22431000292578268 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 1896 0.21253961296715837 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 1761 0.19740625444892718 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 1702 0.19079241628170018 No Hit GTACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT 1684 0.188774635145936 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1637 0.18350598440255184 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 1626 0.18227289593069596 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 1528 0.17128719863597996 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 1494 0.1674758342684254 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA 1478 0.16568225103663506 No Hit GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1328 0.14886740823860037 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 1243 0.13933899731971405 No Hit ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA 1223 0.1370970182799761 No Hit GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC 1207 0.13530343504818573 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA 1199 0.13440664343229053 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 1197 0.13418244552831676 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 1077 0.12073057128988902 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 1064 0.11927328491405934 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA 1035 0.11602241530643931 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1018 0.11411673312266203 No Hit GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT 1013 0.11355623836272755 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 986 0.1105295666590813 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 973 0.10907228028325164 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 964 0.10806338971536954 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 954 0.10694240019550057 No Hit GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA 952 0.10671820229152679 No Hit GTACATGGGCTTGATACCACTGCTTCCAAAAAAAAAAAAAAAAAAAAAAA 940 0.10537301486768401 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 932 0.10447622325178883 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 931 0.10436412429980192 No Hit TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC 922 0.10335523373191985 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAA 904 0.10133745259615567 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 895 0.1003285620282736 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA 894 0.10021646307628669 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 1.1209895198689788E-4 0.0 3 0.0 0.0 0.0 1.1209895198689788E-4 0.0 4 0.0 0.0 0.0 2.2419790397379576E-4 0.0 5 0.0 0.0 0.0 3.3629685596069363E-4 0.0 6 0.0 0.0 0.0 3.3629685596069363E-4 0.0 7 0.0 0.0 0.0 3.3629685596069363E-4 0.0 8 0.0 0.0 0.0 3.3629685596069363E-4 0.0 9 0.0 0.0 0.0 3.3629685596069363E-4 0.0 10-11 0.0 0.0 0.0 7.286431879148362E-4 0.0 12-13 0.0 0.0 0.0 0.0012330884718558767 0.0 14-15 0.0 0.0 0.0 0.0017375337557969171 0.0 16-17 0.0 0.0 0.0 0.0031387706556331406 0.0 18-19 0.0 0.0 0.0 0.005100502315403854 0.0 20-21 0.0 0.0 0.0 0.021522998781484393 0.0 22-23 0.0 0.0 0.0 0.07437765464330674 0.0 24-25 0.0 0.0 0.0 0.16164668876510674 0.0 26-27 0.0 0.0 0.0 0.31572669827109784 0.0 28-29 0.0 0.0 0.0 0.7580131133354034 0.0 30-31 0.0 0.0 0.0 1.4816678978868225 0.0 32-33 0.0 0.0 0.0 2.4730710292589473 0.0 34-35 0.0 0.0 0.0 3.95311349234196 0.0 36-37 0.0 0.0 0.0 5.650740021231542 0.0 38-39 0.0 0.0 0.0 7.416186416073197 0.0 40-41 0.0 0.0 0.0 9.15786783309363 0.0 42-43 0.0 0.0 0.0 10.547950887207154 0.0 44-45 0.0 0.0 0.0 11.877108160915803 0.0 46-47 0.0 0.0 0.0 13.344315293996317 0.0 48-49 0.0 0.0 0.0 14.640627574772804 0.0 50-51 0.0 0.0 0.0 15.705455519696347 0.0 52-53 0.0 0.0 0.0 16.584815748557567 0.0 54-55 0.0 0.0 0.0 17.52207508612002 0.0 56-57 0.0 0.0 0.0 18.59979441052206 0.0 58-59 0.0 0.0 0.0 19.373389278183637 0.0 60-61 0.0 0.0 0.0 20.066272900414653 0.0 62-63 0.0 0.0 0.0 20.681303800490767 0.0 64-65 0.0 0.0 0.0 21.300874708122354 0.0 66-67 0.0 0.0 0.0 21.975990646463444 0.0 68-69 0.0 0.0 0.0 22.757040094432156 0.0 70-71 0.0 0.0 0.0 23.57844516511615 0.0 72-73 0.0 0.0 0.0 24.219090675721272 0.0 74-75 0.0 0.0 0.0 24.886695984279243 0.0 76-77 0.0 0.0 0.0 25.473870294786614 0.0 78-79 0.0 0.0 0.0 26.229473280654297 0.0 80-81 0.0 0.0 0.0 26.922356902885316 0.0 82-83 0.0 0.0 0.0 27.539573732525177 0.0 84-85 0.0 0.0 0.0 28.167664160507762 0.0 86-87 0.0 0.0 0.0 28.716500629435615 0.0 88 0.0 0.0 0.0 29.183729061317006 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACCCATG 675 0.0 52.267265 3 GTACCCA 695 0.0 52.113926 1 ATAACGC 60 1.5952282E-6 47.040535 3 TACCCAT 780 0.0 45.834366 2 CGTATCT 90 6.203536E-7 36.544 94 TATAACG 65 1.4060865E-4 36.185024 2 GTATATG 345 0.0 31.358597 1 ATATGGG 335 0.0 30.892292 3 TAACGCA 155 1.5043042E-9 30.348732 4 TATATGG 355 0.0 29.15188 2 TATGGGA 295 0.0 28.7027 4 CATTAAA 645 0.0 28.409622 94 ATGGGCA 625 0.0 27.84487 5 TATCACG 105 7.3763265E-5 26.880306 2 TAGTCAA 35 0.008836429 26.857689 68-69 GTATAAC 110 1.0125235E-4 25.657032 1 CAAGCCA 130 1.0844644E-5 25.316727 6 TCATTAA 775 0.0 24.250397 94 CGCAGAA 1065 0.0 23.834145 9 ATCATTA 1035 0.0 23.60606 94 >>END_MODULE