##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR523028_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 892069 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.12575036235986 34.0 33.0 35.0 29.0 36.0 2 31.786293436942657 33.0 31.0 34.0 30.0 34.0 3 32.847149715997304 34.0 31.0 35.0 30.0 35.0 4 33.76049946809047 35.0 32.0 36.0 30.0 36.0 5 34.57513488306398 36.0 35.0 36.0 31.0 36.0 6 34.846389685102835 36.0 35.0 37.0 31.0 37.0 7 34.9399418654835 36.0 35.0 36.0 32.0 38.0 8 34.8417162797945 36.0 35.0 37.0 31.0 37.0 9 34.99495330518155 36.0 35.0 36.0 32.0 37.0 10-11 34.97684988493043 36.0 34.5 36.5 31.5 37.5 12-13 34.957024064282024 36.0 35.0 37.0 31.5 37.0 14-15 35.55683528964688 37.0 35.5 37.5 31.5 38.0 16-17 35.64815894286204 37.0 36.0 37.0 31.5 37.5 18-19 35.64624036929879 36.5 35.5 37.5 31.5 39.0 20-21 35.699040657168894 37.0 36.0 37.5 31.0 38.0 22-23 35.718456195653026 37.0 36.0 37.5 31.5 37.5 24-25 35.71048708115627 36.5 35.5 37.5 32.0 38.5 26-27 35.63021862658606 37.0 36.5 37.0 29.5 38.0 28-29 35.34239784142258 37.0 35.0 37.0 31.5 38.0 30-31 35.1755172525892 37.0 35.0 37.0 30.0 37.5 32-33 35.25218677030588 36.5 36.0 37.0 29.5 38.5 34-35 35.070814028959646 37.0 36.0 37.0 30.0 38.5 36-37 34.558409158932776 36.0 35.0 37.0 28.5 38.0 38-39 34.402734541834775 37.0 35.0 37.0 28.0 38.5 40-41 34.32340491598744 37.0 35.0 37.5 27.5 38.0 42-43 33.68138395124144 36.0 34.0 37.5 26.5 38.0 44-45 33.10462699634221 36.5 34.0 37.0 20.0 38.0 46-47 32.269493727503146 36.0 32.5 37.0 14.5 37.5 48-49 31.664727167965708 36.5 31.5 37.0 12.5 38.0 50-51 31.12002546888189 36.5 30.0 37.0 10.0 38.0 52-53 30.738593090893193 36.5 28.0 37.0 8.0 37.5 54-55 30.751580875470395 36.5 27.5 37.0 8.0 37.5 56-57 30.395834290845215 36.0 27.0 37.0 8.0 38.5 58-59 29.9298456733728 36.0 25.5 37.0 8.0 38.0 60-61 29.297784140016077 35.5 24.0 37.0 8.0 37.0 62-63 29.3256368061215 36.5 25.5 37.0 7.5 37.0 64-65 28.820637753357644 36.5 24.0 37.0 7.5 37.5 66-67 28.287400414093526 35.5 18.0 37.0 7.5 37.5 68-69 27.491196308805712 35.0 13.0 37.0 7.0 37.5 70-71 26.88555145397946 35.0 12.0 37.0 7.0 37.5 72-73 26.50601298778458 33.5 12.0 37.0 6.0 37.5 74-75 26.395378608605387 35.5 12.5 36.5 7.0 37.0 76-77 26.437593392439375 34.5 12.5 36.5 7.5 37.0 78-79 26.395761426526423 33.5 12.0 37.0 7.0 37.0 80-81 26.331259689553164 33.0 12.0 36.5 7.5 37.5 82-83 26.266058455119506 34.5 12.5 37.0 7.0 37.0 84-85 26.42058517894916 34.0 12.0 36.5 7.0 37.0 86-87 26.320018406647915 34.0 12.0 37.0 7.0 38.0 88-89 26.130749415123717 33.5 12.5 37.0 6.5 37.5 90-91 26.081245957431545 34.0 12.0 36.5 7.0 38.0 92-93 26.10268488199904 34.0 11.5 37.0 7.0 37.5 94-95 25.96048567991938 34.0 11.5 37.0 7.0 37.0 96-97 25.748244250164504 33.0 11.5 36.5 7.0 37.0 98-99 25.92161648930744 33.0 12.5 37.0 7.5 37.5 100 25.840644613813506 32.0 11.0 37.0 7.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1330563200058208 1101 2 0.2753756484396881 1101 3 -5.127548723109498E-4 1101 4 -0.017423065355664846 1101 5 0.14145062570976563 1101 6 0.20295015974105013 1101 7 0.10381467186702764 1101 8 0.2754822830852248 1101 9 -0.013534341356653101 1101 10-11 0.11660170523914104 1101 12-13 0.14584028016793127 1101 14-15 0.06865640605089851 1101 16-17 -0.0697034292871308 1101 18-19 0.15697461753103426 1101 20-21 0.08167373027282565 1101 22-23 0.15172160658780598 1101 24-25 0.08647294705271236 1101 26-27 0.06826513478360141 1101 28-29 -0.0879192159734643 1101 30-31 0.10087780735830876 1101 32-33 -0.003390912851870098 1101 34-35 -0.053144436952408114 1101 36-37 0.008384028881764038 1101 38-39 0.1617530317504574 1101 40-41 -0.02498711470737902 1101 42-43 -0.02609641557254605 1101 44-45 0.33943977790390534 1101 46-47 0.35207103730765255 1101 48-49 0.2952221023172896 1101 50-51 -0.03456127900856032 1101 52-53 0.06285951591862826 1101 54-55 -0.5019460860744722 1101 56-57 0.08901972065099528 1101 58-59 0.019356991074019447 1101 60-61 0.29168284622260643 1101 62-63 0.1138922800634603 1101 64-65 -0.18566765034731603 1101 66-67 0.163604431001108 1101 68-69 0.24411272285778907 1101 70-71 0.7085299392856221 1101 72-73 0.47506617540068774 1101 74-75 0.6157806571708804 1101 76-77 0.3882996699951562 1101 78-79 0.6979238231565432 1101 80-81 0.5088201111201975 1101 82-83 0.39369447521703194 1101 84-85 0.4335180346033525 1101 86-87 0.6144663728045998 1101 88-89 0.46843386784616214 1101 90-91 0.57164475804937 1101 92-93 0.36596862822982956 1101 94-95 0.5609731127749207 1101 96-97 0.23607209092687853 1101 98-99 0.4256267990725071 1101 100 0.3207172204916695 1102 1 0.1339242635824789 1102 2 0.3285417495642271 1102 3 0.2919617957320284 1102 4 0.06450716263726974 1102 5 0.13703238627512349 1102 6 0.5271488192659888 1102 7 0.23155100771491988 1102 8 0.36525485102038857 1102 9 0.08706158113135842 1102 10-11 -0.6172501975063085 1102 12-13 0.21967297317670642 1102 14-15 0.19097545594012644 1102 16-17 0.1112557955929816 1102 18-19 0.19092327004512555 1102 20-21 0.2635745126840021 1102 22-23 0.18025998399603793 1102 24-25 0.19202694464098613 1102 26-27 0.21474708884849036 1102 28-29 0.011170775333077643 1102 30-31 0.13840129371518373 1102 32-33 0.06790034601686301 1102 34-35 0.24860056430954813 1102 36-37 0.09822319991852879 1102 38-39 0.2143246125575473 1102 40-41 0.07997376473399243 1102 42-43 0.07134462315540446 1102 44-45 0.09514030231569137 1102 46-47 0.2176105925391454 1102 48-49 0.01837824803084942 1102 50-51 -0.21570488931731902 1102 52-53 0.1678778843529649 1102 54-55 -0.08680965487898007 1102 56-57 0.04704644602187358 1102 58-59 -0.152736309355042 1102 60-61 -0.3723066094547818 1102 62-63 -0.4007765272502617 1102 64-65 -0.6881445846416021 1102 66-67 -0.19962224389162486 1102 68-69 -0.5454768618192922 1102 70-71 -0.20158952564608867 1102 72-73 -0.32015242298961866 1102 74-75 -0.2753050434937485 1102 76-77 0.051281666125166936 1102 78-79 -0.06544797638915156 1102 80-81 -0.31700015865741804 1102 82-83 -0.21829127873007792 1102 84-85 -0.20707129768302224 1102 86-87 -0.11562070198717933 1102 88-89 -0.21509343241464407 1102 90-91 -0.09772061955516165 1102 92-93 -0.13394724153657123 1102 94-95 -0.14020681375134458 1102 96-97 -1.0561633895969713 1102 98-99 -0.36762245076624467 1102 100 -0.6474787468658008 1103 1 0.2427752624897579 1103 2 0.1937624575616681 1103 3 -0.05115512487082441 1103 4 -0.11739086428890033 1103 5 -0.07163649331307909 1103 6 0.16359330036338093 1103 7 -0.006600584282828947 1103 8 0.18788987703252502 1103 9 0.1235857823835147 1103 10-11 0.017551086263082993 1103 12-13 0.08905356131902664 1103 14-15 -0.03458035160441142 1103 16-17 -0.011655501989977779 1103 18-19 0.14556044025453474 1103 20-21 0.10200443798071035 1103 22-23 -0.00396504675807563 1103 24-25 -0.04037586205825505 1103 26-27 -0.005238616503071114 1103 28-29 0.04382030216646626 1103 30-31 0.09277662445074242 1103 32-33 0.022275676752414597 1103 34-35 -0.10765437524745636 1103 36-37 -0.10422956932598026 1103 38-39 -0.0499882478153566 1103 40-41 -0.39982679743054206 1103 42-43 -0.17273924794535134 1103 44-45 -0.23298991643365952 1103 46-47 -0.0068421904502145026 1103 48-49 -0.4020624119970506 1103 50-51 -0.3291349864247124 1103 52-53 -0.5531812941170919 1103 54-55 -0.4369811219360038 1103 56-57 -0.3505227765004477 1103 58-59 -0.8901903499985764 1103 60-61 -0.39555121975380203 1103 62-63 -0.20343340758068962 1103 64-65 -0.6073647016103116 1103 66-67 -0.8117163411639581 1103 68-69 0.0025861445926800286 1103 70-71 -0.050315721276600556 1103 72-73 0.09665134859838886 1103 74-75 0.1297805525862188 1103 76-77 0.005879663499896992 1103 78-79 0.08429327672921261 1103 80-81 0.04376996552734269 1103 82-83 0.16759069420630368 1103 84-85 -0.17805659756138326 1103 86-87 0.04861175098823978 1103 88-89 0.21713344014994007 1103 90-91 -0.036872836943778964 1103 92-93 -0.19069974481815422 1103 94-95 -0.10853866951284274 1103 96-97 -0.16574286578057595 1103 98-99 -0.2294550521860863 1103 100 -0.5284524323422986 1104 1 0.11166094997300746 1104 2 0.30529840636895855 1104 3 0.06476135194504451 1104 4 0.15592728098046393 1104 5 0.17246138035796577 1104 6 0.19408668908847915 1104 7 0.22731654025930226 1104 8 0.4530137267600338 1104 9 0.059731699474554034 1104 10-11 0.2244842699493148 1104 12-13 0.32953798796960854 1104 14-15 0.27200356244900803 1104 16-17 0.032548325178780146 1104 18-19 0.2612542611693911 1104 20-21 0.3202867907905045 1104 22-23 0.14165051654041605 1104 24-25 0.2399374475995657 1104 26-27 0.21390574269464224 1104 28-29 0.19240228420881778 1104 30-31 0.11170935584537034 1104 32-33 0.15238629276242222 1104 34-35 0.29397778324445767 1104 36-37 0.37320470879427603 1104 38-39 0.2554118906640568 1104 40-41 0.2577012055623982 1104 42-43 -0.04538984430021742 1104 44-45 -0.09541997874939767 1104 46-47 -0.26099517669162253 1104 48-49 0.09473734566398662 1104 50-51 -0.22531102837057304 1104 52-53 -0.411763387836384 1104 54-55 -0.23630114896182164 1104 56-57 -0.6277963794219161 1104 58-59 -0.4746020637929149 1104 60-61 -0.3881812396322992 1104 62-63 -0.38581720772363326 1104 64-65 -0.35319635978952846 1104 66-67 -0.6132964302821584 1104 68-69 -0.2637738292749141 1104 70-71 -0.2534756203206463 1104 72-73 0.001528420205648473 1104 74-75 -0.20920851982510769 1104 76-77 -0.3648703309162542 1104 78-79 -0.1655496775725851 1104 80-81 -0.29788958173433855 1104 82-83 -0.5143156870141006 1104 84-85 -0.8001648627717834 1104 86-87 -0.7646406724013808 1104 88-89 -0.17163004484067912 1104 90-91 -0.2031570100877076 1104 92-93 -0.30152305514604194 1104 94-95 -0.27200230191702346 1104 96-97 0.015240086734390701 1104 98-99 -0.677681622363874 1104 100 -0.42506750426224826 1105 1 0.04499971572703032 1105 2 0.30016612692523736 1105 3 0.1020644266951507 1105 4 0.11757439093265987 1105 5 0.18769932038404136 1105 6 0.2525123735734809 1105 7 0.2545777853701239 1105 8 0.26550557812900166 1105 9 0.23408795762318135 1105 10-11 0.1484670491757356 1105 12-13 0.05593479913866162 1105 14-15 0.14350779973714367 1105 16-17 0.006943289324809143 1105 18-19 0.16336000323805422 1105 20-21 0.28444503720510994 1105 22-23 0.16156577077770606 1105 24-25 0.08638057015497225 1105 26-27 0.06529033091715775 1105 28-29 -0.008853963074969329 1105 30-31 0.04946671677539172 1105 32-33 0.03037819845560108 1105 34-35 -0.0842680881158202 1105 36-37 -0.026668987424770307 1105 38-39 0.12598175159408953 1105 40-41 -0.004795399174668091 1105 42-43 -0.21497236277044607 1105 44-45 0.026721888916831915 1105 46-47 -0.0037279559180447563 1105 48-49 0.058775399770901515 1105 50-51 0.267449592011868 1105 52-53 0.16326784858256715 1105 54-55 0.48042866959533015 1105 56-57 0.46058570988708425 1105 58-59 0.6078627612831369 1105 60-61 0.19064929600438418 1105 62-63 0.37129247285002975 1105 64-65 0.48177484838083373 1105 66-67 0.5800019997482622 1105 68-69 0.4657724277872326 1105 70-71 0.2954351793316796 1105 72-73 0.4029211570591862 1105 74-75 0.28621670290413803 1105 76-77 0.37475306651964146 1105 78-79 0.3594876703196377 1105 80-81 0.09690683830043056 1105 82-83 0.45273803962648884 1105 84-85 0.2660407271891039 1105 86-87 0.2151819590632016 1105 88-89 0.23645271808848278 1105 90-91 0.7652453149883485 1105 92-93 0.44496828014296597 1105 94-95 -0.32872016914866364 1105 96-97 0.08070686748189004 1105 98-99 0.19272254973521186 1105 100 0.0866502271588736 1106 1 0.13357681005111743 1106 2 0.3552564849901181 1106 3 -0.02839166639423496 1106 4 0.023774114059087026 1106 5 0.25351291712141943 1106 6 0.26229648701681896 1106 7 0.23748645450619676 1106 8 0.27789379977752304 1106 9 0.10481044798873285 1106 10-11 0.1635098897489371 1106 12-13 0.2066513030485737 1106 14-15 0.27262442337616477 1106 16-17 -0.05319814068978701 1106 18-19 0.23045133410197138 1106 20-21 0.1393801215767212 1106 22-23 0.07514941278765974 1106 24-25 -0.016204830376324253 1106 26-27 0.21692912314973256 1106 28-29 0.337935800085873 1106 30-31 0.13990148311655304 1106 32-33 -0.3434029965906049 1106 34-35 0.10901959849322651 1106 36-37 0.07756081764127032 1106 38-39 0.16972777621338508 1106 40-41 0.1846160574048099 1106 42-43 0.2646718172722089 1106 44-45 0.3683034356673147 1106 46-47 0.4195294242593093 1106 48-49 0.2937325591976929 1106 50-51 0.13363547145961974 1106 52-53 0.4126523661116579 1106 54-55 0.4119585831658732 1106 56-57 0.44886535219635704 1106 58-59 0.6314232057774731 1106 60-61 0.292336728189067 1106 62-63 0.4477787975404155 1106 64-65 0.28147739636601443 1106 66-67 0.2050553166188429 1106 68-69 0.14797768838512937 1106 70-71 0.3695026017883727 1106 72-73 0.338750094089157 1106 74-75 0.3271715425793751 1106 76-77 0.27475306651964004 1106 78-79 0.4699452600229357 1106 80-81 0.427447115922309 1106 82-83 0.10789574531736434 1106 84-85 0.16147044245830955 1106 86-87 0.3211309993595286 1106 88-89 0.30342806137430145 1106 90-91 0.3364878786879757 1106 92-93 0.1511867109432785 1106 94-95 0.283581997199974 1106 96-97 0.22479524253333594 1106 98-99 0.30219611579200745 1106 100 0.2790100131430826 1107 1 0.1732239025243345 1107 2 0.336943723261367 1107 3 0.08621893989972307 1107 4 0.1881489352951391 1107 5 0.18433952159656997 1107 6 0.2294442578762812 1107 7 0.2148833581313525 1107 8 0.2905557874125577 1107 9 0.20928926400304704 1107 10-11 0.3029952696085729 1107 12-13 0.35893984822175895 1107 14-15 0.2178438171555399 1107 16-17 0.20407525996880338 1107 18-19 0.24059595871602113 1107 20-21 0.1887208034832355 1107 22-23 0.23675018431450212 1107 24-25 0.1505865126817696 1107 26-27 0.14175109259668517 1107 28-29 0.07723196518675479 1107 30-31 0.1498627600961555 1107 32-33 0.1696165189316048 1107 34-35 0.26745028942045224 1107 36-37 0.1312321706734778 1107 38-39 -0.09687801903864823 1107 40-41 0.3103668659678789 1107 42-43 0.37636536270208154 1107 44-45 0.34547482170209065 1107 46-47 0.6283440451330691 1107 48-49 0.5437820630589982 1107 50-51 0.6067292061042586 1107 52-53 0.791752935956108 1107 54-55 0.9616494590541933 1107 56-57 0.4792992260385134 1107 58-59 0.6531205648381473 1107 60-61 0.7222539283973468 1107 62-63 0.5138774532355619 1107 64-65 0.6188865476131475 1107 66-67 0.614694268457086 1107 68-69 0.7109025965279656 1107 70-71 0.6276817397611332 1107 72-73 0.8309651535304603 1107 74-75 0.5985297085163168 1107 76-77 0.6828372304177606 1107 78-79 0.825940360974144 1107 80-81 0.4193743195775248 1107 82-83 0.7618564209167289 1107 84-85 0.4915676900436168 1107 86-87 0.8891163022131536 1107 88-89 0.6370345826829613 1107 90-91 0.7978785338257453 1107 92-93 0.6386090632200911 1107 94-95 0.7892951094893981 1107 96-97 0.7533479696460503 1107 98-99 0.4943517705997529 1107 100 0.6535965642273993 1108 1 0.23688021069004606 1108 2 0.316415192113805 1108 3 0.017011982988080376 1108 4 0.1324182538563079 1108 5 0.2545344102202023 1108 6 0.32656829257953746 1108 7 0.2901842030470121 1108 8 0.30905302255878553 1108 9 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0.147989758058916 2316 90-91 0.3177137663321332 2316 92-93 0.24027778258061616 2316 94-95 0.3770193102325088 2316 96-97 0.5395624916788755 2316 98-99 0.3626171235423463 2316 100 0.5180739207716982 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 3.0 13 7.0 14 23.0 15 53.0 16 201.0 17 1170.0 18 3779.0 19 9786.0 20 18539.0 21 28718.0 22 40392.0 23 46355.0 24 38633.0 25 29712.0 26 29591.0 27 31961.0 28 38127.0 29 44766.0 30 41889.0 31 30465.0 32 25770.0 33 31144.0 34 46942.0 35 97647.0 36 209827.0 37 45867.0 38 702.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.61042200879867 20.576266015178945 14.184327951380709 19.62898402464167 2 14.044512404977013 22.998898008006538 41.00071635084824 21.95587323616821 3 21.817307444395993 33.147398121441555 35.037951185280924 9.997343248881531 4 9.956303779532503 25.023765004461545 30.48657725644233 34.53335395956362 5 7.025541103216012 42.36031543520327 34.81301024469323 15.80113321688749 6 20.112659951909333 37.09045865491865 15.501448885451172 27.29543250772085 7 14.66718084044993 40.85058393849644 17.910511341343383 26.57172387971024 8 24.09521727676795 29.66846586291358 22.687248126537867 23.549068733780608 9 22.59488896038311 17.82406966277272 18.7191797943881 40.861861582456065 10-11 21.675267540710525 27.354478760128938 24.019839461561183 26.950414237599357 12-13 29.099563202761246 24.82323247892662 24.56103082578948 21.516173492522654 14-15 25.605054771609336 25.813334857880786 26.11028531457243 22.47132505593744 16-17 18.26584191268788 33.40539468020231 26.648536427879616 21.68022697923019 18-19 18.852571115973742 28.713803045521395 30.50134295297198 21.932282885532878 20-21 20.425169143400986 24.86647606097514 29.058586868609304 25.649767927014572 22-23 17.69550208307994 31.02301000427658 29.4381541327824 21.843333779861076 24-25 22.445686382542966 31.242359717262598 28.09583836146377 18.216115538730662 26-27 22.415657251562255 27.700170224071375 32.1227451756301 17.761427348736266 28-29 17.837833596208323 28.789855827921695 32.64139479563571 20.730915780234266 30-31 18.305805413064533 29.163826810632422 33.42546050507587 19.104907271227177 32-33 12.339682329716599 27.064470095520804 39.52779923256025 21.068048342202346 34-35 13.004379745331319 27.680057753897998 34.952150133230795 24.363412367539887 36-37 14.442901213808812 29.611027410466466 38.15286502822654 17.793206347498174 38-39 16.275558161264087 26.3441803424327 36.087767786529696 21.29249370977352 40-41 14.172380974800994 28.68711598672285 36.45481969241945 20.685683346056706 42-43 18.06633974527484 27.594515836667842 35.248601690806495 19.090542727250817 44-45 15.171584632070424 28.55173882703092 35.09232920708143 21.184347333817236 46-47 19.475734152106373 26.819861523776535 32.476614691841924 21.227789632275172 48-49 17.8541101614403 25.761955286815347 32.350163413254776 24.03377113848958 50-51 13.169058963785691 26.856690295376623 36.223277576901665 23.750973163936013 52-53 15.38426615130753 28.164122753524413 34.97579770061341 21.475813394554653 54-55 14.404147691445388 26.573306242555876 31.28766201919709 27.734884046801657 56-57 20.219086437356793 27.192097463422073 26.550666541115696 26.038149558105435 58-59 16.563460204043885 30.46107730059424 31.60421405214654 21.371248443215332 60-61 17.32184384148402 35.524400503776924 25.92926608759598 21.224489567143074 62-63 11.940710099965528 38.81274469414808 26.125501999590835 23.121043206295557 64-65 12.096111527755825 39.54909326526517 25.041225406284646 23.31356980069436 66-67 14.55128887792793 36.075466198833034 23.869043949083856 25.504200974155182 68-69 15.15356353149881 33.68459992769524 25.290297990880635 25.871538549925315 70-71 16.797151748320733 32.38669993812048 25.088391311756215 25.727757001802576 72-73 16.22604622016655 28.920701771692713 28.119631435901198 26.733620572239538 74-75 15.659698206524848 29.256154526509782 26.313438340393713 28.770708926571658 76-77 15.742887249736565 26.79780508037576 28.058594713360087 29.400712956527585 78-79 16.59256817957617 25.56352964387886 28.940447684898707 28.903454491646265 80-81 17.4613948847872 24.15937536783458 28.19852137504274 30.180708372335474 82-83 16.643125306177815 23.71069013179799 29.2166780449909 30.429506517033296 84-85 17.063871955662474 24.061672566828015 28.075164842063256 30.79929063544626 86-87 17.458498072454198 23.770019516471297 27.14650356979913 31.624978841275375 88-89 17.77474734056715 22.84441470057244 26.444787096839285 32.93605086202113 90-91 19.203543989892253 21.288491225788658 26.79237075793536 32.715594026383734 92-93 19.109465398932592 21.608456294568775 27.556621967080773 31.725456339417857 94-95 18.448178540018084 20.830042472425436 28.026980107972 32.69479887958448 96-97 17.495971233909458 21.29380144559134 28.63167689155823 32.57855042894097 98-99 18.725925643589665 21.699655167775003 26.65673050621729 32.91768868241804 100 18.239672664088335 20.188218149206882 26.901182669132893 34.670926517571885 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 8961.0 1 5114.5 2 1238.5 3 1061.0 4 737.5 5 755.0 6 1284.0 7 2348.5 8 3129.0 9 2763.0 10 1662.0 11 869.5 12 705.5 13 671.0 14 683.5 15 734.5 16 826.0 17 902.5 18 1017.5 19 1181.5 20 1379.0 21 1716.0 22 2159.5 23 2683.0 24 3448.5 25 4474.5 26 5749.0 27 7236.0 28 8822.5 29 10647.0 30 12801.5 31 14847.0 32 16632.0 33 18075.5 34 19157.5 35 20097.0 36 20422.0 37 20303.0 38 20384.0 39 20336.5 40 20312.5 41 20229.0 42 20260.5 43 20624.5 44 21206.0 45 22170.5 46 25593.5 47 40981.5 48 65151.0 49 69767.0 50 74659.5 51 81553.5 52 65285.0 53 37459.5 54 14187.5 55 6274.0 56 4366.0 57 3435.5 58 2914.0 59 2663.0 60 2473.0 61 1945.0 62 1321.0 63 958.0 64 741.5 65 528.5 66 360.0 67 256.5 68 188.5 69 171.0 70 155.0 71 102.5 72 73.5 73 51.5 74 36.0 75 24.0 76 13.0 77 8.5 78 8.5 79 7.0 80 4.5 81 4.5 82 2.0 83 1.0 84 2.0 85 1.0 86 0.5 87 0.5 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06400850158451869 2 0.005380749695371098 3 2.2419790397379573E-4 4 1.1209895198689786E-4 5 0.0106494004387553 6 4.4839580794759146E-4 7 0.002578275895698651 8 4.4839580794759146E-4 9 0.0 10-11 9.52841091888632E-4 12-13 8.407421399017341E-4 14-15 1.1209895198689786E-4 16-17 5.604947599344894E-4 18-19 5.604947599344894E-4 20-21 5.604947599344893E-5 22-23 5.604947599344893E-5 24-25 5.044452839410405E-4 26-27 0.0010649400438755298 28-29 0.0011209895198689788 30-31 5.604947599344894E-4 32-33 0.0013451874238427745 34-35 8.967916158951829E-4 36-37 1.1209895198689786E-4 38-39 0.0011770389958624277 40-41 0.0011209895198689788 42-43 0.0010649400438755298 44-45 0.0018496327077838148 46-47 2.802473799672447E-4 48-49 6.165442359279383E-4 50-51 5.044452839410405E-4 52-53 5.604947599344894E-4 54-55 7.286431879148362E-4 56-57 1.1209895198689786E-4 58-59 6.725937119213873E-4 60-61 8.407421399017341E-4 62-63 0.0012891379478493256 64-65 0.0024661769437117533 66-67 0.00156938532781657 68-69 0.0012891379478493256 70-71 0.0014572863758296724 72-73 0.0014012368998362235 74-75 2.2419790397379573E-4 76-77 0.001008890567882081 78-79 0.0014572863758296724 80-81 0.002690374847685549 82-83 0.0024661769437117533 84-85 3.3629685596069363E-4 86-87 2.2419790397379573E-4 88-89 9.52841091888632E-4 90-91 0.00896791615895183 92-93 0.021186701925523698 94-95 0.008799767730971483 96-97 0.005212601267390751 98-99 0.004596057031462813 100 0.0021298800877510597 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 892069.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 26.336253231632195 #Duplication Level Percentage of deduplicated Percentage of total 1 80.35958249711503 21.163703142322596 2 9.067462490675714 4.77605976645524 3 3.212958246649008 2.538518460192278 4 1.701042490008136 1.7919634309848178 5 1.0728365271641984 1.4127247227770592 6 0.733610369012261 1.1592329070994827 7 0.5414819128033586 0.9982423343156485 8 0.4048814514169126 0.8530448346645279 9 0.3346656932104808 0.7932456399897859 >10 2.1334365326141134 10.537471266022227 >50 0.22306501225744962 4.084371634578172 >100 0.16431888306992687 8.406197610643424 >500 0.02383900894278732 4.476204932015863 >1k 0.020007739648410787 13.276926540950221 >5k 0.003831269294376533 6.624393147773914 >10k+ 0.002979876117848415 17.10769962921476 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT 54734 6.135624038050868 No Hit ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT 28775 3.225647343422986 RNA PCR Primer, Index 27 (95% over 23bp) GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 17481 1.9596017796829617 No Hit ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCT 15467 1.7338344903813494 RNA PCR Primer, Index 27 (96% over 25bp) GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12437 1.3941746658610488 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12177 1.3650289383444554 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 11523 1.2917162237450244 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 7632 0.8555392015640045 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 7630 0.8553150036600309 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 7521 0.8430962178934589 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6770 0.7589099049512986 No Hit CATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCT 6525 0.7314456617145086 RNA PCR Primer, Index 27 (96% over 28bp) TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6465 0.7247197245952948 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 6237 0.6991611635422821 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 5163 0.5787668891083537 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 5144 0.5766370090206027 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 4871 0.5460339951281795 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 4707 0.5276497670023284 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 4635 0.5195786424592717 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 4356 0.4883030348549271 No Hit TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTG 4288 0.4806803061198181 RNA PCR Primer, Index 27 (96% over 30bp) TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 4254 0.4768689417522636 No Hit CACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTG 4105 0.46016619790621577 RNA PCR Primer, Index 27 (100% over 24bp) ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 3921 0.4395399907406266 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 3797 0.4256397206942512 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 3486 0.390776946626326 No Hit GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA 3432 0.3847236032190335 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 3329 0.373177411164383 No Hit TTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC 3251 0.36443369290940497 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 3218 0.36073442749383733 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 3143 0.35232700609482004 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 3078 0.34504057421567164 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 3046 0.34145340775209093 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 2847 0.31914571630669825 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 2665 0.2987437070450828 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 2662 0.29840741018912215 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2625 0.2942597489656069 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 2522 0.28271355691095645 No Hit TATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCT 2429 0.27228835437617493 RNA PCR Primer, Index 27 (95% over 22bp) TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 2258 0.25311943358641537 No Hit CTTTACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC 2243 0.25143794930661195 RNA PCR Primer, Index 27 (95% over 21bp) TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 2229 0.24986856397879537 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 2168 0.24303052790759458 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 1882 0.21097022763934178 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 1839 0.20614997270390517 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 1739 0.1949400775052154 No Hit ACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGC 1714 0.19213760370554295 TruSeq Adapter, Index 27 (96% over 25bp) CTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC 1667 0.18686895296215877 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 1611 0.18059141165089249 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 1585 0.17767683889923314 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 1577 0.17678004728333796 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA 1573 0.17633165147539037 No Hit GTACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT 1564 0.17532276090750826 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1558 0.1746501671955869 No Hit GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1294 0.14505604387104584 No Hit ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA 1264 0.1416930753114389 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA 1256 0.14079628369554373 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 1239 0.13889060151176646 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 1199 0.13440664343229053 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 1105 0.12386934194552215 No Hit TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTG 1084 0.12151526395379729 TruSeq Adapter, Index 1 (96% over 28bp) GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC 1071 0.12005797757796761 No Hit GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT 1039 0.11647081111438688 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 975 0.10929647818722543 No Hit GTACATGGGCTTGATACCACTGCTTCCAAAAAAAAAAAAAAAAAAAAAAA 942 0.1055972127716578 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 938 0.1051488169637102 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 937 0.10503671801172332 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 921 0.10324313477993294 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 920 0.10313103582794605 No Hit TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC 918 0.10290683792397225 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA 916 0.10268264001999845 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 903 0.10122535364416878 No Hit GGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 894 0.10021646307628669 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 1.1209895198689788E-4 0.0 3 0.0 0.0 0.0 1.1209895198689788E-4 0.0 4 0.0 0.0 0.0 2.2419790397379576E-4 0.0 5 0.0 0.0 0.0 3.3629685596069363E-4 0.0 6 0.0 0.0 0.0 3.3629685596069363E-4 0.0 7 0.0 0.0 0.0 3.3629685596069363E-4 0.0 8 0.0 0.0 0.0 3.3629685596069363E-4 0.0 9 0.0 0.0 0.0 3.3629685596069363E-4 0.0 10-11 0.0 0.0 0.0 6.725937119213873E-4 0.0 12-13 0.0 0.0 0.0 0.0011209895198689788 0.0 14-15 0.0 0.0 0.0 0.0016814842798034682 0.0 16-17 0.0 0.0 0.0 0.0031387706556331406 0.0 18-19 0.0 0.0 0.0 0.004988403363416955 0.0 20-21 0.0 0.0 0.0 0.02157904825747784 0.0 22-23 0.0 0.0 0.0 0.07348086302741155 0.0 24-25 0.0 0.0 0.0 0.16030150134126397 0.0 26-27 0.0 0.0 0.0 0.31365286765934025 0.0 28-29 0.0 0.0 0.0 0.7548182932037768 0.0 30-31 0.0 0.0 0.0 1.4744375154836677 0.0 32-33 0.0 0.0 0.0 2.4611885403483362 0.0 34-35 0.0 0.0 0.0 3.9354018579280305 0.0 36-37 0.0 0.0 0.0 5.626582697078365 0.0 38-39 0.0 0.0 0.0 7.381715988337225 0.0 40-41 0.0 0.0 0.0 9.111514916447046 0.0 42-43 0.0 0.0 0.0 10.493807093397484 0.0 44-45 0.0 0.0 0.0 11.816070281558938 0.0 46-47 0.0 0.0 0.0 13.272459865772714 0.0 48-49 0.0 0.0 0.0 14.559131636678329 0.0 50-51 0.0 0.0 0.0 15.613702527495072 0.0 52-53 1.1209895198689788E-4 0.0 0.0 16.482077059061574 0.0 54-55 1.1209895198689788E-4 0.0 0.0 17.40476353286573 0.0 56-57 1.1209895198689788E-4 0.0 0.0 18.469087032505332 0.0 58-59 1.1209895198689788E-4 0.0 0.0 19.22698804688875 0.0 60-61 1.1209895198689788E-4 0.0 0.0 19.90630769592935 0.0 62-63 1.1209895198689788E-4 0.0 0.0 20.505756841679286 0.0 64-65 1.1209895198689788E-4 0.0 0.0 21.111203281360524 0.0 66-67 1.6814842798034682E-4 0.0 0.0 21.77723920459068 0.0 68-69 2.2419790397379576E-4 0.0 0.0 22.54859209321252 0.0 70-71 2.2419790397379576E-4 0.0 0.0 23.363775672061244 0.0 72-73 2.2419790397379576E-4 0.0 0.0 23.998031542403112 0.0 74-75 2.2419790397379576E-4 0.0 0.0 24.66384326772929 0.0 76-77 2.2419790397379576E-4 0.0 0.0 25.25096152876067 0.0 78-79 2.2419790397379576E-4 0.0 0.0 26.009703285283983 0.0 80-81 3.3629685596069363E-4 0.0 0.0 26.70062517585523 0.0 82-83 3.3629685596069363E-4 0.0 0.0 27.31761780759112 0.0 84-85 3.3629685596069363E-4 0.0 0.0 27.94335415758198 0.0 86-87 3.3629685596069363E-4 0.0 0.0 28.48888370742622 0.0 88 3.3629685596069363E-4 0.0 0.0 28.958298068871354 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACCCA 655 0.0 61.762634 1 ACCCATG 680 0.0 59.445255 3 CTATACA 365 0.0 59.283466 1 GCTCGCA 25 0.005233075 56.40703 94 CTTTACA 420 0.0 54.880165 1 CGGAGAT 45 1.6055243E-5 52.266827 1 TACCCAT 850 0.0 47.556202 2 CGTCAGA 50 3.007661E-5 47.003223 2 GAGTAGG 45 0.0010095831 41.81346 1 GCGTCAG 50 0.0016947014 37.632114 1 CTAGTTA 50 0.0017026559 37.596256 9 GATGTGT 220 0.0 36.32067 3 ATGTGTA 225 0.0 35.513546 4 ATAACGC 55 0.0027157704 34.184162 3 CTTATCA 155 4.1836756E-11 33.383327 1 GAGATGT 240 0.0 33.320103 1 TGTATAA 250 0.0 31.956818 7 ATACGTG 30 0.004154491 31.344269 92-93 GTGTATA 255 0.0 31.330212 6 ATGGGTG 110 2.9658768E-6 29.911142 5 >>END_MODULE