FastQCFastQC Report
Mon 6 Jun 2016
ERR523027_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523027_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42362845
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAT92900.013.5018581
TTGCGCG41300.011.0336758
CAACGGT86850.010.8182781
CGCGCTA29400.010.2271559
CGAAGAT192800.09.9414821
CCGGAAT109500.09.6959931
CGGGAAT106200.09.6433951
CGGAGAT171750.09.6281731
CGACAAT54150.09.543161
CGGCGGT103600.09.4772981
CCGGGAT122000.09.4726891
CGGCGCT174000.09.4226741
CGAAAAT79650.09.2600181
CTCGAAT150600.09.0151071
CGGCGAT60800.08.8857121
CTCGGAT184850.08.8187691
CGAGGAT116700.08.8159931
CGCCAAC108300.08.7623571
TGCGCGG84950.08.7365767
CGAGAAT121050.08.7320391
Produced by FastQC (version 0.11.5)