FastQCFastQC Report
Mon 6 Jun 2016
ERR523027_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523027_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42362845
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAT95800.015.5133411
CCGGGAT116800.010.7108161
CGGGAAT104300.010.4613431
CGAAGAT201000.010.1315421
CGAAAAT77450.010.01950551
GACGATC153850.09.9264672
CGCAGAT118650.09.7510441
CAACGGT85800.09.7021891
CTCGAAT146600.09.6243421
TGCGCGG87600.09.605287
CGGAAAT93450.09.5622041
TTGCGCG45400.09.5257298
CGAGGAT118900.09.3745461
CGACAAT51650.09.2878161
ACGATCA107000.09.2214073
CGGCGAT64100.09.0980311
CCGACAT109550.08.9296551
CGCGCTA28250.08.8190889
AGATCGA135250.08.789114
CGGCAAT77850.08.6993671
Produced by FastQC (version 0.11.5)