FastQCFastQC Report
Mon 6 Jun 2016
ERR523026_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523026_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6791996
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT494980.7287695693578147No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT199790.2941550613398477No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158620.23353959572414354No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC105410.15519738233061386No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC104740.15421092709713022No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91170.13423152781597633No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA80780.11893411009075976No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG240300.027.0015181
TACATGG248000.025.49642
ACATGGG251200.024.3303323
CATGGGG163300.022.1084164
GAGTACT174450.020.01035912-13
GTATAGG18100.019.7423061
ATGGGGG91550.019.1019125
GAGTACA187500.018.9576021
GTACTTT188300.018.34164214-15
AGAGTAC281350.018.19947410-11
AGTACAT181600.017.9401342
AGTACTT186200.016.81734812-13
CATGGGA93850.016.2791484
TACCTGG48150.015.7194682
ACTTTTT231950.015.01125616-17
GTATAGC21900.014.5991531
ATGGGGA73050.014.3508255
TATCACG5652.1827873E-1114.1451892
GTGTAGC33450.014.0561611
TACTTTT222700.013.85210314-15
Produced by FastQC (version 0.11.5)