##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR523026_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6791996 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.05916758490435 34.0 31.0 34.0 30.0 34.0 2 32.36220074334555 34.0 31.0 34.0 30.0 35.0 3 32.23092048346318 34.0 31.0 34.0 30.0 35.0 4 34.837589421430756 37.0 35.0 37.0 32.0 37.0 5 34.844521404311784 37.0 35.0 37.0 33.0 37.0 6 35.01134953554154 37.0 35.0 37.0 33.0 37.0 7 34.870649364340025 37.0 35.0 37.0 32.0 37.0 8 35.0164003335691 37.0 35.0 37.0 33.0 37.0 9 36.290719546949084 39.0 35.0 39.0 33.0 39.0 10-11 36.39160299858834 39.0 35.5 39.0 33.0 39.0 12-13 36.44643106385811 39.0 36.5 39.0 33.0 39.0 14-15 37.57172420301779 40.0 37.0 41.0 32.5 41.0 16-17 37.460482161650276 40.0 36.5 41.0 33.0 41.0 18-19 37.48241393251704 40.0 36.5 41.0 32.5 41.0 20-21 37.50808061429954 40.0 36.0 41.0 33.0 41.0 22-23 37.413150051914045 39.5 36.0 41.0 33.0 41.0 24-25 37.414632237710386 39.0 36.5 41.0 32.0 41.0 26-27 37.24313434519102 39.0 36.5 41.0 32.0 41.0 28-29 37.06373568241206 39.0 36.0 41.0 32.0 41.0 30-31 36.98681926491123 39.0 36.5 41.0 31.5 41.0 32-33 36.82744424466681 38.0 35.5 41.0 31.0 41.0 34-35 36.7301235159738 38.5 35.5 41.0 31.0 41.0 36-37 36.61662411167498 38.0 36.0 41.0 30.5 41.0 38-39 36.3785573489737 38.0 35.5 41.0 30.0 41.0 40-41 36.337169515411965 38.0 35.5 40.0 30.0 41.0 42-43 36.29299752237781 38.0 35.5 40.0 30.0 41.0 44-45 36.19253544907859 38.0 35.0 40.0 30.0 41.0 46-47 35.91244311686874 38.0 35.0 40.0 29.0 41.0 48-49 36.02900988163127 38.0 35.5 40.0 29.0 41.0 50-51 35.00712677098161 37.5 34.0 39.0 27.0 40.0 52-53 35.15331693363777 37.5 34.5 39.5 28.0 40.0 54-55 35.730866302041406 37.5 35.0 40.0 29.0 41.0 56-57 35.868101954123645 37.5 35.0 40.0 28.5 41.0 58-59 35.85586497695228 38.0 35.0 40.0 29.0 41.0 60-61 35.58413506132807 37.0 35.0 40.0 28.0 41.0 62-63 35.380711943882176 37.5 35.0 40.0 28.0 41.0 64-65 35.14426355669232 37.0 35.0 39.5 28.0 41.0 66-67 34.90251967168414 37.0 35.0 39.0 27.5 41.0 68-69 34.561924359201626 37.0 34.0 39.0 27.5 41.0 70-71 34.16029669334317 36.0 34.0 38.5 26.0 40.5 72-73 33.904519672862 36.0 34.0 38.0 26.0 40.0 74-75 33.64625899367432 35.0 34.0 37.0 26.0 39.0 76-77 33.30878191035448 35.0 33.5 37.0 25.5 39.0 78-79 33.0152795290221 35.0 33.0 37.0 25.0 38.5 80-81 32.78755317288173 35.0 33.0 36.0 25.0 38.5 82-83 32.56233940656031 35.0 32.5 36.0 24.0 38.0 84-85 32.27265585845457 35.0 33.0 36.0 23.5 37.0 86-87 32.186489141041896 35.0 32.5 36.0 23.5 37.0 88-89 31.885098430564447 35.0 32.0 35.5 21.0 37.0 90-91 31.78754058453509 35.0 32.0 35.5 20.5 37.0 92-93 31.549254961280894 35.0 31.5 35.0 20.0 36.0 94-95 31.456313431279995 35.0 31.5 35.0 20.0 36.0 96-97 31.195748716577572 35.0 31.0 35.0 19.0 36.0 98-99 31.14954926357436 35.0 31.0 35.0 18.5 36.5 100 29.91278749280771 34.0 29.0 35.0 17.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.050706464022006514 1101 2 0.0832722232578007 1101 3 0.06942632767955814 1101 4 0.030350870069788982 1101 5 0.030732687446921148 1101 6 0.01991263400422838 1101 7 0.05785221552119424 1101 8 0.04427485949224774 1101 9 -0.0799298474154071 1101 10-11 -0.05615689083241193 1101 12-13 -0.002511623147533726 1101 14-15 0.041778784356999665 1101 16-17 -0.10563985521147146 1101 18-19 -0.02204493413223929 1101 20-21 -0.028572215880920737 1101 22-23 -0.0607163949682743 1101 24-25 -0.14102326398145948 1101 26-27 -0.13778699075378853 1101 28-29 -0.18157090743781623 1101 30-31 -0.12751818951638683 1101 32-33 -0.11291399320481332 1101 34-35 -0.26761163628723494 1101 36-37 -0.1641052132383507 1101 38-39 -0.2524560791739887 1101 40-41 -0.24848903089614538 1101 42-43 -0.24776905643982872 1101 44-45 -0.31842174417450053 1101 46-47 -0.2854247245395811 1101 48-49 -0.34338134459790837 1101 50-51 -0.5316650708724566 1101 52-53 -0.4164362385567557 1101 54-55 -0.30584280072687875 1101 56-57 -0.3051949591316472 1101 58-59 -0.26322316451692274 1101 60-61 -0.21896586481413038 1101 62-63 -0.25040491390559794 1101 64-65 -0.24089914419348446 1101 66-67 -0.433054515193696 1101 68-69 -0.28670165008885107 1101 70-71 -0.2738804490840394 1101 72-73 -0.23482292273892114 1101 74-75 -0.367262305923056 1101 76-77 -0.3422205337908011 1101 78-79 -0.7309441099334677 1101 80-81 -0.3818780538268527 1101 82-83 -0.4022970286511125 1101 84-85 -0.7044420747917925 1101 86-87 -0.88001072906507 1101 88-89 -0.44744327620126256 1101 90-91 -0.5548971630083734 1101 92-93 -0.3906331310804241 1101 94-95 -0.565135122999731 1101 96-97 -0.5805671443683664 1101 98-99 -0.5623212355536857 1101 100 -0.6549311323790015 1102 1 -0.06059796196844047 1102 2 -0.09598563518007808 1102 3 0.015339834500977645 1102 4 0.003558762370829527 1102 5 4.278914668915945E-4 1102 6 0.03730381871273636 1102 7 0.13444301884346999 1102 8 -3.7189751338928545E-5 1102 9 -0.10027952713420518 1102 10-11 0.03449078815650353 1102 12-13 -0.04706018138627144 1102 14-15 -0.04347044037894676 1102 16-17 -0.07074414996854728 1102 18-19 -0.07026158315932918 1102 20-21 -0.06395039809809333 1102 22-23 -0.07935615409324015 1102 24-25 -0.07846232376073914 1102 26-27 -0.14810857671697164 1102 28-29 -0.3895827585701568 1102 30-31 -0.22760967222301787 1102 32-33 -0.22554244764009468 1102 34-35 -0.15297209867447492 1102 36-37 -0.15618917874710547 1102 38-39 -0.28081931837495233 1102 40-41 -0.5881339945260606 1102 42-43 -0.06799213482430133 1102 44-45 -0.1492714922846261 1102 46-47 -0.2307960882625082 1102 48-49 -0.4815007518303318 1102 50-51 -0.33908036395133934 1102 52-53 -0.4370668672971405 1102 54-55 -0.14700250884900612 1102 56-57 -0.05815163687048397 1102 58-59 -0.11218812809639189 1102 60-61 -0.5332097531926152 1102 62-63 -0.1843340141773595 1102 64-65 -0.1343715624268711 1102 66-67 -0.16258218398295554 1102 68-69 -0.39037230913466914 1102 70-71 -0.07404390032676389 1102 72-73 -0.39453555078060987 1102 74-75 -0.20275822669322707 1102 76-77 -0.39853352274144527 1102 78-79 -0.24879964476333782 1102 80-81 -0.33031153716247275 1102 82-83 -0.3321593734908106 1102 84-85 -0.34760541355859687 1102 86-87 -0.39130637253223455 1102 88-89 -0.39982742743860555 1102 90-91 -0.4025288570023342 1102 92-93 -0.48266754297870307 1102 94-95 -0.5672463108910364 1102 96-97 -0.4954824830223501 1102 98-99 -0.5301814540137926 1102 100 -0.7549466909744247 1103 1 -0.04244091226130564 1103 2 0.02748066833737539 1103 3 -0.0374246411601149 1103 4 -0.0019360495468490058 1103 5 0.014394275053859928 1103 6 -0.032974342672027035 1103 7 0.1499208272603454 1103 8 0.017713679799044257 1103 9 -0.02364835471902893 1103 10-11 -0.029186734521204016 1103 12-13 0.05555955849452232 1103 14-15 8.208568104350888E-6 1103 16-17 -0.07137280124976542 1103 18-19 -0.14356534498842421 1103 20-21 -0.12043709611457842 1103 22-23 -0.14230186536335765 1103 24-25 -0.1632851270110507 1103 26-27 -0.21668089786661682 1103 28-29 -0.25836106896525735 1103 30-31 -0.15921591237198385 1103 32-33 -0.3402884104072541 1103 34-35 -0.3266747014302567 1103 36-37 -0.38051435480587514 1103 38-39 -0.2261078808069712 1103 40-41 -0.19074936282814292 1103 42-43 -0.2600475038934107 1103 44-45 -0.2725094350311039 1103 46-47 -0.3259633251177192 1103 48-49 -0.26194824397661165 1103 50-51 -0.39706201633276095 1103 52-53 -0.2512056485991536 1103 54-55 -0.12367333365176592 1103 56-57 -0.19306409172650518 1103 58-59 -0.06537508316854712 1103 60-61 0.021733104110040813 1103 62-63 -0.06691827236211623 1103 64-65 -0.14453067761933625 1103 66-67 -0.09518981242205626 1103 68-69 -0.03283695727337488 1103 70-71 -0.058831468424365596 1103 72-73 -0.05857683902892319 1103 74-75 -0.07560466203603511 1103 76-77 -0.24900265705586122 1103 78-79 -0.2208856194490494 1103 80-81 -0.2285026395273846 1103 82-83 -0.15886603268356225 1103 84-85 -0.24187414831299492 1103 86-87 -0.390475875303391 1103 88-89 -0.17887957557874046 1103 90-91 -0.2934002244972156 1103 92-93 -0.35882535217630007 1103 94-95 -0.49313663768309723 1103 96-97 -0.3916604106132553 1103 98-99 -0.351989730724906 1103 100 -0.36514689672397793 1104 1 -0.03503607596189795 1104 2 -0.0010132615601818884 1104 3 0.08154049349922587 1104 4 -0.058788616975157026 1104 5 -0.05923891231756073 1104 6 -0.04127563795843514 1104 7 0.06567223427833113 1104 8 -0.09359782087565804 1104 9 -0.051855093763037985 1104 10-11 -0.14433912440429708 1104 12-13 -0.06849968444920762 1104 14-15 -0.06716701939048164 1104 16-17 -0.0970168698378373 1104 18-19 -0.2081123151045503 1104 20-21 -0.20729346077881416 1104 22-23 -0.1344972265455695 1104 24-25 -0.1447739186040664 1104 26-27 -0.14218241626325323 1104 28-29 -0.25371942088884225 1104 30-31 -0.1594759995038899 1104 32-33 -0.12344951972502116 1104 34-35 -0.20618554803197497 1104 36-37 -0.2726144163163937 1104 38-39 -0.31685927425048277 1104 40-41 -0.1655829351176621 1104 42-43 -0.23984658884642585 1104 44-45 -0.10680087813731376 1104 46-47 -0.16753825993983185 1104 48-49 -0.22500547545315897 1104 50-51 -0.24892227053942406 1104 52-53 -0.25433958994271677 1104 54-55 -0.21460190806025992 1104 56-57 -0.6786884067240635 1104 58-59 -0.09067438246268722 1104 60-61 -0.07070164990174277 1104 62-63 -0.06518125864202062 1104 64-65 -0.05584288869208365 1104 66-67 -0.07677488104967267 1104 68-69 -0.14769683962965274 1104 70-71 -0.18961862203858715 1104 72-73 -0.1577347300173031 1104 74-75 -0.069323238800159 1104 76-77 -0.2738202091907098 1104 78-79 -0.15931059051050767 1104 80-81 -0.2169020656705527 1104 82-83 -0.1339330861144532 1104 84-85 -0.28310682738330684 1104 86-87 -0.328256488187769 1104 88-89 -0.3094098320650147 1104 90-91 -0.5276598399610144 1104 92-93 -0.6044327393386162 1104 94-95 -0.370050153643934 1104 96-97 -0.3968618817616374 1104 98-99 -0.4142401581628157 1104 100 -0.40512812140134 1105 1 0.02035939166081846 1105 2 0.05458382883705326 1105 3 0.1280687308650741 1105 4 0.09916453502420808 1105 5 0.10718904361993253 1105 6 0.0504678589965053 1105 7 0.14047272492125984 1105 8 0.09095523397068916 1105 9 0.012408200091108768 1105 10-11 0.011127186254675792 1105 12-13 0.02501277957914283 1105 14-15 0.026456937722819873 1105 16-17 0.04668169412022394 1105 18-19 0.03559790703258159 1105 20-21 0.0268725498574085 1105 22-23 0.03193391556355607 1105 24-25 0.01674201509641904 1105 26-27 -0.056456359724158744 1105 28-29 -0.08752497236426393 1105 30-31 0.05326298399825902 1105 32-33 0.020828400261450497 1105 34-35 -0.14284062803651665 1105 36-37 -0.08447909598557857 1105 38-39 0.05134922900436578 1105 40-41 -0.043623459736636505 1105 42-43 -0.0915865961411555 1105 44-45 -0.11636347316087381 1105 46-47 0.08049523034569006 1105 48-49 0.006813885394393537 1105 50-51 -0.09572905265644494 1105 52-53 -0.07227757118070599 1105 54-55 -0.05460066659942697 1105 56-57 0.016757114508855864 1105 58-59 0.07825811067489497 1105 60-61 0.021407041919445646 1105 62-63 0.0899607272515297 1105 64-65 0.02383157350507048 1105 66-67 0.05338598348384238 1105 68-69 0.10603659709151714 1105 70-71 0.11458255843113108 1105 72-73 0.07470348163361251 1105 74-75 0.004373667431771366 1105 76-77 0.09265325169585026 1105 78-79 0.08777965543994526 1105 80-81 0.10270730326941191 1105 82-83 0.06000406937786806 1105 84-85 0.02207358511543589 1105 86-87 0.15377403102709053 1105 88-89 0.12373534751862181 1105 90-91 -0.007906263517742929 1105 92-93 -0.030239546761638536 1105 94-95 0.005568307483766688 1105 96-97 0.0020062342051048176 1105 98-99 0.013485205945542589 1105 100 0.06050949893279878 1106 1 0.06362553297346807 1106 2 0.020551784643174642 1106 3 0.07858149669250025 1106 4 0.0850231572642528 1106 5 0.09794042253145818 1106 6 0.0841827590470885 1106 7 0.25496625723567234 1106 8 0.051828806231867475 1106 9 0.013829458209521306 1106 10-11 0.05801107754901125 1106 12-13 0.1538670555549828 1106 14-15 0.1547550086113958 1106 16-17 0.04290441654256938 1106 18-19 0.011701648915291685 1106 20-21 -0.016465750403774848 1106 22-23 -0.024643365287609242 1106 24-25 4.941108985079268E-4 1106 26-27 0.006712319006936696 1106 28-29 0.05216859166007026 1106 30-31 0.008326653619043611 1106 32-33 0.06342053720009488 1106 34-35 0.07180300478332668 1106 36-37 0.0490496611821456 1106 38-39 0.04540220300300035 1106 40-41 0.021086749521394665 1106 42-43 0.12661791610099726 1106 44-45 -0.01664751794164232 1106 46-47 0.04616228592199434 1106 48-49 0.06768960927151824 1106 50-51 -0.055934207550535575 1106 52-53 0.026740075622342374 1106 54-55 -0.04514565225734657 1106 56-57 0.0726922268081509 1106 58-59 0.1588710212968607 1106 60-61 0.18795959649911254 1106 62-63 0.15652278761198346 1106 64-65 0.1853575530422802 1106 66-67 0.1126803820563751 1106 68-69 0.13033952636036616 1106 70-71 0.12932068923399953 1106 72-73 0.1082537279812712 1106 74-75 0.1367206770835594 1106 76-77 0.1192553186231109 1106 78-79 0.14685926338628974 1106 80-81 0.1606632370956902 1106 82-83 0.0710425224195177 1106 84-85 0.1313304844169778 1106 86-87 0.09918118308842594 1106 88-89 0.19694396761604693 1106 90-91 0.1253539913154249 1106 92-93 0.11452683968234467 1106 94-95 0.07193607040437655 1106 96-97 0.0952321220884258 1106 98-99 0.0917329915235463 1106 100 0.09860524193030074 1107 1 -0.1004877408255922 1107 2 -0.0805631263591593 1107 3 -0.047045198908975294 1107 4 0.014620908547286149 1107 5 -0.03430889328274134 1107 6 -0.02531451166824894 1107 7 0.09923285697006179 1107 8 0.006248595702189164 1107 9 0.01897481215609531 1107 10-11 -0.024340636433976215 1107 12-13 -0.021503487887116535 1107 14-15 0.02931354419543908 1107 16-17 0.011901746818111292 1107 18-19 -0.008284986248234816 1107 20-21 0.002864998167595445 1107 22-23 -0.09700543691077002 1107 24-25 -0.08115291170691563 1107 26-27 -0.025626110129699953 1107 28-29 0.009615380391664985 1107 30-31 -0.015291803431985329 1107 32-33 0.004904475075008463 1107 34-35 0.060294803957951615 1107 36-37 -0.006120068133434131 1107 38-39 0.039727995429494456 1107 40-41 -0.014585678812316871 1107 42-43 0.026664775180663014 1107 44-45 0.054745430085745284 1107 46-47 0.09261144863077675 1107 48-49 -0.03227959453057139 1107 50-51 -0.13789536434610028 1107 52-53 -0.07911025074817957 1107 54-55 -0.015654201364043274 1107 56-57 -0.05422784424902716 1107 58-59 0.010089897842050277 1107 60-61 0.054700448601479934 1107 62-63 -0.01482630637014637 1107 64-65 0.014800945004047605 1107 66-67 0.13335252177707702 1107 68-69 0.11383009433564695 1107 70-71 0.09364902837310041 1107 72-73 0.04382973578658067 1107 74-75 0.013930679913478627 1107 76-77 0.05767175412912451 1107 78-79 0.02438604872803296 1107 80-81 0.05167905979087095 1107 82-83 0.03408684710937848 1107 84-85 0.05275926585822788 1107 86-87 0.0655644901439203 1107 88-89 0.19422750298930325 1107 90-91 0.04707855245608883 1107 92-93 -0.0186990258378259 1107 94-95 0.07552898898675409 1107 96-97 -0.006872223515610898 1107 98-99 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2316 64-65 0.07376810953508794 2316 66-67 0.10038386003125765 2316 68-69 0.1484423211016619 2316 70-71 0.21207898715538676 2316 72-73 0.1882458489923593 2316 74-75 0.1532388287534232 2316 76-77 -1.1662007113744366 2316 78-79 0.22553673881073877 2316 80-81 -0.7880908137511113 2316 82-83 0.2358197210297135 2316 84-85 -1.280343571144165 2316 86-87 0.28360935727092595 2316 88-89 0.46334808387473103 2316 90-91 -0.4480208817153901 2316 92-93 0.3820322630229107 2316 94-95 0.42074177379853595 2316 96-97 0.5540698890598392 2316 98-99 0.49882065744246873 2316 100 0.494555452632909 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 15560.0 1 1239.0 2 839.0 3 632.0 4 624.0 5 656.0 6 746.0 7 1016.0 8 1570.0 9 2095.0 10 3086.0 11 5368.0 12 8663.0 13 11296.0 14 13422.0 15 15296.0 16 17045.0 17 20399.0 18 24776.0 19 30276.0 20 36918.0 21 44954.0 22 53375.0 23 59266.0 24 61550.0 25 64125.0 26 67510.0 27 74180.0 28 84636.0 29 100970.0 30 123273.0 31 154593.0 32 202264.0 33 280924.0 34 438972.0 35 822172.0 36 1122481.0 37 1146549.0 38 1317406.0 39 360796.0 40 478.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.76665591664673 18.01869559277705 12.498535764667212 23.716112725909014 2 15.962528637027921 20.80658921566256 38.75623111637652 24.474651030933007 3 20.486654275499447 24.797825861443737 29.35562876504355 25.359891098013264 4 12.96995064326884 15.806078671100824 37.80502185012825 33.41894883550209 5 13.475954779613497 37.82678621753271 34.365726282151414 14.331532720702386 6 31.58984057261303 36.15006695725782 17.42188270695168 14.838209763177465 7 27.75835514840328 32.23870253126695 21.872946413286407 18.129995907043355 8 26.01219875661179 33.64234408244514 20.210094620265036 20.135362540678027 9 26.269332531356277 16.245190501062343 20.504167247666743 36.981309719914634 10-11 25.385379021721516 25.312975205421644 28.16220492731376 21.139440845543078 12-13 26.470164078865782 23.200615604959545 27.84380021016875 22.485420106005922 14-15 23.184526986987493 24.89694733759764 26.37499163839225 25.543534037022614 16-17 22.56284795403195 27.795982092791483 26.723482439236623 22.917687513939942 18-19 22.33150474299465 27.686871744376525 28.03112534625297 21.95049816637585 20-21 23.421543277830935 26.071068796844475 27.785597114350946 22.721790810973637 22-23 22.871793326792407 26.002119413933535 27.94355190352568 23.182535355748378 24-25 23.33725351136052 26.092191480930815 27.79101488107062 22.779540126638047 26-27 22.975253855410237 25.746222783748735 28.668986987678945 22.609536373162083 28-29 22.38214827867011 27.17766506759484 27.998060256996744 22.442126396738303 30-31 23.076557270303745 26.084106825939017 27.83457613481478 23.00475976894245 32-33 22.95176909325742 26.319143090042168 28.003956186869722 22.725131629830695 34-35 23.559541236601824 26.154071319873722 27.908475939998866 22.377911503525585 36-37 23.210473501794688 25.94008832981268 28.395389492172995 22.45404867621963 38-39 22.779576462344338 26.676928498977336 28.145620629384833 22.397874409293493 40-41 22.94214799595202 26.64857353312609 27.798439005521836 22.61083946540006 42-43 22.554863391795745 26.789499496748785 27.264569526911366 23.391067584544096 44-45 22.903136865957652 26.167168674698793 27.53455231004426 23.395142149299293 46-47 23.40477489237171 26.591425875730334 27.374606701963216 22.629192529934734 48-49 23.135117336881763 26.60544956487622 27.42584164897967 22.833591449262347 50-51 22.26606050150276 26.86957956412591 28.080723321514895 22.783636612856437 52-53 22.94426721877744 27.29429670221375 26.90691936579363 22.854516713215183 54-55 22.507522338001014 26.485485378259277 27.442345979529193 23.564646304210516 56-57 23.05716782192551 26.399898815268347 27.220909186481702 23.322024176324444 58-59 23.670991397567022 26.373823867072034 27.089715772092333 22.86546896326862 60-61 23.039734807341453 26.59488599851566 27.98649162340179 22.378887570741096 62-63 22.592612428598073 26.74884436458058 27.741403605323956 22.917139601497386 64-65 22.430862199813646 27.249038498046268 27.304299098944174 23.01580020319591 66-67 22.787109447271195 26.570477470470284 26.92469299265696 23.717720089601556 68-69 22.398054195541146 26.692276458406017 27.270594563427185 23.639074782625652 70-71 23.086480973783857 26.402463657316222 27.141093925790027 23.369961443109897 72-73 22.807329023090734 26.17996936327614 27.752131423019154 23.260570190613976 74-75 22.230305779345237 27.45173290229784 27.140468344784562 23.17749297357236 76-77 22.511583362503018 26.452545052852216 27.032834047304306 24.00303753734046 78-79 22.6049621125995 26.48089142096995 27.310352836158803 23.603793630271745 80-81 22.988355524105586 26.455825455428794 27.031854397543746 23.52396462292187 82-83 22.757078696995617 26.199788262120233 27.62642930272569 23.416703738158457 84-85 22.500861417819852 26.68491404734577 27.31178390026348 23.5024406345709 86-87 22.327613000334594 26.873417122080173 27.16459167336677 23.634378204218468 88-89 22.125755538386407 26.71437766197015 26.818904439699164 24.340962359944275 90-91 22.644051553344717 26.07162511477957 26.9594089774417 24.32491435443401 92-93 22.987557574039013 26.40593592894069 27.071219521130836 23.53528697588946 94-95 22.684464829820616 26.117050046215066 27.211731349906238 23.98675377405808 96-97 21.909403417644594 26.136007551254508 27.926608012679015 24.027981018421883 98-99 22.35586777881612 26.440572330286845 26.94317028199462 24.260389608902415 100 22.290772896910624 25.362385025517696 27.05100761363253 25.295834463939144 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 9863.0 1 7686.0 2 4143.5 3 2326.0 4 1601.0 5 1491.5 6 2126.0 7 3373.5 8 5189.0 9 6417.5 10 6120.0 11 5604.0 12 5827.0 13 6215.5 14 6511.5 15 6883.5 16 7263.5 17 7743.0 18 8349.0 19 8887.0 20 9655.5 21 10837.0 22 12407.5 23 14947.0 24 18344.5 25 22601.5 26 28607.5 27 35409.0 28 42920.0 29 52014.0 30 61917.0 31 72489.0 32 84688.5 33 97173.0 34 109560.5 35 122037.0 36 138104.0 37 153944.0 38 165783.5 39 177061.0 40 185900.5 41 192901.0 42 200587.0 43 209709.0 44 218565.0 45 229453.5 46 243223.5 47 280422.0 48 351887.0 49 351694.0 50 288611.5 51 260316.0 52 249459.0 53 242549.0 54 235532.0 55 222592.5 56 204214.5 57 184752.0 58 166123.0 59 146086.0 60 125570.5 61 105596.5 62 85900.0 63 67132.0 64 50818.5 65 38410.0 66 28315.5 67 21423.5 68 16342.5 69 12170.5 70 9246.5 71 6927.0 72 5203.5 73 4141.5 74 3233.5 75 2350.0 76 1674.0 77 1102.0 78 693.5 79 483.0 80 337.5 81 245.5 82 190.0 83 154.0 84 113.5 85 77.0 86 69.5 87 72.0 88 54.5 89 29.5 90 27.0 91 24.0 92 23.0 93 22.0 94 14.5 95 9.5 96 8.5 97 7.0 98 4.5 99 5.5 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2019141354029066 2 0.18127219156195026 3 0.3652829006377507 4 0.3611604011545354 5 0.37139303380037325 6 0.3020025335703967 7 0.3213930043539484 8 0.311278157407631 9 0.32479406642760095 10-11 0.2981965831546426 12-13 0.30580848398615074 14-15 0.29406672206520734 16-17 0.2891565307164492 18-19 0.31088799227796954 20-21 0.31489270606166436 22-23 0.3060146089603115 24-25 0.30985000580094574 26-27 0.2999928150723293 28-29 0.3243082004170792 30-31 0.30725872041149616 32-33 0.306677153520114 34-35 0.3080684970956991 36-37 0.3159895853884484 38-39 0.3228064327481936 40-41 0.3486824786115893 42-43 0.3168508933161916 44-45 0.3021792121196773 46-47 0.3075752694789573 48-49 0.330212208605541 50-51 0.32073045979414594 52-53 0.3502505007364551 54-55 0.35072164353453683 56-57 0.3559557455569762 58-59 0.3400620377279374 60-61 0.3235352317639763 62-63 0.34648871995802116 64-65 0.3409748768992208 66-67 0.3275546687601112 68-69 0.33819955135427054 70-71 0.3582746515162848 72-73 0.3625959143674407 74-75 0.3503903712546356 76-77 0.3341653911456956 78-79 0.3443317693355532 80-81 0.35748695965074184 82-83 0.3631701196526029 84-85 0.3545938484062712 86-87 0.35427729933880997 88-89 0.34445691664129363 90-91 0.3553079242096138 92-93 0.33535797135334 94-95 0.3560293616191765 96-97 0.366733137063096 98-99 0.35719249540194076 100 0.37856323825867977 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 6791996.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.40826579889233 #Duplication Level Percentage of deduplicated Percentage of total 1 73.87418300558808 41.67114550655227 2 14.775515033056832 16.669223586003984 3 5.059494157751738 8.561918737752087 4 2.2423407013350065 5.0594620117031885 5 1.1681395472348126 3.2946363035309525 6 0.7152467992988492 2.4207498939993894 7 0.45038158160792835 1.778367077638588 8 0.3075330369587355 1.3877924232567147 9 0.22257452176443768 1.129953850537477 >10 1.1105501528361936 10.839478433396254 >50 0.05156045542576586 1.963667179188629 >100 0.020862833561276686 2.003293361133755 >500 8.765128921748453E-4 0.3357336923234652 >1k 4.7196589245238074E-4 0.4172359723730901 >5k 1.011355483761736E-4 0.44488562842431284 >10k+ 1.6855924729362266E-4 2.0224563421859103 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 49498 0.7287695693578147 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19979 0.2941550613398477 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15862 0.23353959572414354 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 10541 0.15519738233061386 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 10474 0.15421092709713022 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9117 0.13423152781597633 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 8078 0.11893411009075976 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 1.472321244005444E-5 5 0.0 1.472321244005444E-5 0.0 0.0 1.472321244005444E-5 6 0.0 1.472321244005444E-5 0.0 0.0 2.944642488010888E-5 7 0.0 1.472321244005444E-5 0.0 0.0 2.944642488010888E-5 8 0.0 1.472321244005444E-5 0.0 0.0 2.944642488010888E-5 9 0.0 1.472321244005444E-5 0.0 1.472321244005444E-5 5.889284976021776E-5 10-11 0.0 1.472321244005444E-5 0.0 3.68080311001361E-5 5.889284976021776E-5 12-13 0.0 1.472321244005444E-5 0.0 5.889284976021776E-5 5.889284976021776E-5 14-15 0.0 1.472321244005444E-5 0.0 7.36160622002722E-5 7.36160622002722E-5 16-17 2.944642488010888E-5 1.472321244005444E-5 0.0 8.833927464032664E-5 7.36160622002722E-5 18-19 2.944642488010888E-5 2.944642488010888E-5 0.0 1.2514730574046275E-4 1.0306248708038108E-4 20-21 2.944642488010888E-5 2.944642488010888E-5 0.0 1.840401555006805E-4 1.0306248708038108E-4 22-23 2.944642488010888E-5 2.944642488010888E-5 0.0 3.239106736811977E-4 1.3250891196048997E-4 24-25 2.944642488010888E-5 2.944642488010888E-5 0.0 6.03651710042232E-4 1.6195533684059885E-4 26-27 2.944642488010888E-5 4.416963732016332E-5 0.0 0.0011263257516641646 1.6195533684059885E-4 28-29 2.944642488010888E-5 5.889284976021776E-5 0.0 0.003239106736811977 1.766785492806533E-4 30-31 2.944642488010888E-5 5.889284976021776E-5 0.0 0.01022527103961781 2.0612497416076217E-4 32-33 2.944642488010888E-5 5.889284976021776E-5 0.0 0.028003550060983547 2.502946114809255E-4 34-35 2.944642488010888E-5 5.889284976021776E-5 0.0 0.05666964468176954 2.6501782392097993E-4 36-37 2.944642488010888E-5 5.889284976021776E-5 0.0 0.10198769257225711 2.944642488010888E-4 38-39 2.944642488010888E-5 5.889284976021776E-5 0.0 0.16626187647931479 3.165490674611705E-4 40-41 2.944642488010888E-5 5.889284976021776E-5 0.0 0.2306391228734528 3.239106736811977E-4 42-43 2.944642488010888E-5 5.889284976021776E-5 0.0 0.29899163662640554 3.3863388612125213E-4 44-45 2.944642488010888E-5 5.889284976021776E-5 0.0 0.38706589344281117 3.6071870478133377E-4 46-47 2.944642488010888E-5 5.889284976021776E-5 0.0 0.496260598504475 3.68080311001361E-4 48-49 2.944642488010888E-5 5.889284976021776E-5 0.0 0.6077962943441073 3.68080311001361E-4 50-51 2.944642488010888E-5 5.889284976021776E-5 0.0 0.7243010743822582 3.68080311001361E-4 52-53 2.944642488010888E-5 5.889284976021776E-5 0.0 0.8415714614672918 4.1224994832152434E-4 54-55 2.944642488010888E-5 7.36160622002722E-5 0.0 0.9807941583004466 4.637811918617149E-4 56-57 2.944642488010888E-5 7.36160622002722E-5 0.0 1.1549035070103102 4.785044043017693E-4 58-59 2.944642488010888E-5 9.570088086035386E-5 0.0 1.3418220505430214 4.8586601052179654E-4 60-61 2.944642488010888E-5 1.0306248708038108E-4 0.0 1.5358077360469589 4.8586601052179654E-4 62-63 2.944642488010888E-5 1.0306248708038108E-4 0.0 1.7385095633154082 5.300356478419599E-4 64-65 2.944642488010888E-5 1.472321244005444E-4 0.0 1.9525629873751398 5.373972540619871E-4 66-67 2.944642488010888E-5 1.472321244005444E-4 0.0 2.202180331083823 5.594820727220687E-4 68-69 2.944642488010888E-5 1.472321244005444E-4 0.0 2.493677852578241 5.594820727220687E-4 70-71 2.944642488010888E-5 1.472321244005444E-4 0.0 2.8041020636643488 5.594820727220687E-4 72-73 2.944642488010888E-5 1.472321244005444E-4 0.0 3.1124723866150683 5.742052851621231E-4 74-75 2.944642488010888E-5 1.472321244005444E-4 0.0 3.4351021408139815 5.742052851621231E-4 76-77 2.944642488010888E-5 1.472321244005444E-4 0.0 3.786037270928899 5.742052851621231E-4 78-79 2.944642488010888E-5 1.472321244005444E-4 0.0 4.203285749873823 5.742052851621231E-4 80-81 4.416963732016332E-5 1.472321244005444E-4 0.0 4.640484181675019 6.03651710042232E-4 82-83 4.416963732016332E-5 1.472321244005444E-4 0.0 5.0760262520767085 6.330981349223409E-4 84-85 4.416963732016332E-5 1.5459373062057163E-4 0.0 5.518164321651544 6.404597411423681E-4 86-87 4.416963732016332E-5 1.6195533684059885E-4 0.0 5.980635147606094 6.551829535824226E-4 88 4.416963732016332E-5 1.6195533684059885E-4 0.0 6.358734604672912 6.625445598024498E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 24030 0.0 27.001518 1 TACATGG 24800 0.0 25.4964 2 ACATGGG 25120 0.0 24.330332 3 CATGGGG 16330 0.0 22.108416 4 GAGTACT 17445 0.0 20.010359 12-13 GTATAGG 1810 0.0 19.742306 1 ATGGGGG 9155 0.0 19.101912 5 GAGTACA 18750 0.0 18.957602 1 GTACTTT 18830 0.0 18.341642 14-15 AGAGTAC 28135 0.0 18.199474 10-11 AGTACAT 18160 0.0 17.940134 2 AGTACTT 18620 0.0 16.817348 12-13 CATGGGA 9385 0.0 16.279148 4 TACCTGG 4815 0.0 15.719468 2 ACTTTTT 23195 0.0 15.011256 16-17 GTATAGC 2190 0.0 14.599153 1 ATGGGGA 7305 0.0 14.350825 5 TATCACG 565 2.1827873E-11 14.145189 2 GTGTAGC 3345 0.0 14.056161 1 TACTTTT 22270 0.0 13.852103 14-15 >>END_MODULE