FastQCFastQC Report
Mon 6 Jun 2016
ERR523026_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523026_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6791996
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT507920.7478214062552452No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT183750.2705390285860003No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155670.22919624805432748No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT138100.20332756379715183No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC108150.15923154253918878No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC106660.15703778388562067No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89820.13224389413656898No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG235400.026.8735581
TACATGG244300.025.7230832
ACATGGG247450.024.1572553
CATGGGG162300.021.7165414
GAGTACT181000.019.68684212-13
TACCTGG48150.019.4254842
GAGTACA184550.019.063251
ATGGGGG94150.018.7178365
GTACTTT192950.018.24601614-15
AGTACAT180150.018.132832
AGAGTAC285150.018.06021110-11
AGTACTT190200.016.8191112-13
CATGGGA95250.016.6269594
TATACCG5150.016.4251755
GTACCTG60150.016.2643641
GTATACG4451.3278623E-1015.8540631
GTGTACG9200.015.8483191
GTGTAGC33150.015.1813221
ACTTTTT235050.015.05578516-17
GTAATAC15550.014.2041553
Produced by FastQC (version 0.11.5)