FastQCFastQC Report
Mon 6 Jun 2016
ERR523025_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523025_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5490869
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175090.318874844765009No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148520.27048541861042397No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93700.17064694131293243No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA83750.053.986371
GTATCAA150850.040.4832081
TCAACGC184200.032.555674
ATCAACG187500.031.93463
CAACGCA188050.031.8629935
TACCTGG48850.031.4638772
TATCAAC193500.031.2141252
AACGCAG196800.030.6309266
GTACATG177400.027.3963221
ACGCAGA225300.026.673737
TACATGG183550.025.607942
CGCAGAG234150.025.604438
GTACCTG63500.024.7467561
ACATGGG189600.024.1690543
ACCTGGG63300.022.942893
GCAGAGT257650.022.7758739
GTGGTAT42850.022.72821
CATGGGG131000.022.1708724
CAGAGTA249350.020.43304810-11
AGAGTAC234550.020.18006710-11
Produced by FastQC (version 0.11.5)