FastQCFastQC Report
Mon 6 Jun 2016
ERR523025_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523025_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5490869
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163250.297311773418743No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150480.2740549810967991No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91750.16709559088005924No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA84850.056.6103171
GTATCAA148500.039.7276421
TACCTGG53300.032.473682
TCAACGC184200.031.454354
CAACGCA188150.030.7945
ATCAACG188650.030.7129443
TATCAAC193800.029.9968412
AACGCAG195650.029.805426
GTACCTG66100.026.9037021
GTACATG173750.026.1020071
ACCTGGG65100.025.841473
ACGCAGA226900.025.6797337
CGCAGAG237250.024.4875938
TACATGG184500.024.3454512
ACATGGG187300.023.3073623
GTGGTAT42350.022.9952771
GCAGAGT264150.021.869319
TATCACG4850.021.3349592
CATGGGG129900.020.8724734
GAGTACT166100.020.84207212-13
Produced by FastQC (version 0.11.5)