FastQCFastQC Report
Mon 6 Jun 2016
ERR523024_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523024_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10037027
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT1056091.0521940411239303No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT318600.3174246716682141No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT311390.31024126965086374No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC243940.24304009543861943No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT240700.2398120479301291No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA181600.18093007022896324No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG179860.1791964891595888No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA153220.15265476520089066No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136170.1356676633429401No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG401000.027.4485131
TACATGG410200.026.5328882
ACATGGG420500.025.3361423
CATGGGG298200.023.5219334
ATGGGGG178250.021.2844455
GAGTACT262500.020.34997412-13
GTACTTT275700.019.12070814-15
AGAGTAC453500.018.7596610-11
GAGTACA309000.018.6245041
AGTACAT302800.017.8088862
AGTACTT277300.017.71360212-13
ACTTTTT337900.015.96907716-17
CATGGGA132900.015.8122754
GTATAGG25650.015.3976241
TGGGGGG160000.015.298216
TACCTGG64650.015.1259962
TACTTTT324300.014.92238114-15
ATGGGGA113800.014.7070675
CATGGGT63900.014.5672024
GTATATG31850.014.4669971
Produced by FastQC (version 0.11.5)