FastQCFastQC Report
Mon 6 Jun 2016
ERR523024_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523024_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10037027
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT1057111.0532102783025292No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT316500.31533241865345185No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT279100.27807038877149576No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC250520.2495958215515411No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT233600.2327382401183139No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC231240.23038694625410494No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA185620.18493524028579378No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG183430.1827533192846846No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA157730.15714812762783242No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC139580.13906508371453022No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128100.12762743390049663No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG395300.028.0547081
TACATGG409450.026.6206152
ACATGGG414550.025.631163
CATGGGG292200.024.1404384
ATGGGGG173100.021.2029575
GAGTACT267100.020.1539512-13
GTACTTT280050.019.04226114-15
AGAGTAC453400.018.78902210-11
GAGTACA307000.017.617661
AGTACTT282600.017.45978512-13
GTATAGG27100.017.3547951
AGTACAT297900.017.2767412
TACCTGG66350.016.859872
TGGGGGG151300.016.3376396
ACTTTTT338850.015.86647616-17
ATGGGGA109950.015.7287815
CATGGGA133050.015.5411244
GTATATA41900.015.2655921
TACTTTT325250.015.05190814-15
GTACCTG81900.014.5286551
Produced by FastQC (version 0.11.5)