FastQCFastQC Report
Mon 6 Jun 2016
ERR523023_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523023_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5658173
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT214970.3799282913406854No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT174930.30916339956378147No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99510.17586949002796487No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57870.1022768303478879No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG81700.046.4007952
ACCTGGG90850.040.5375023
GTACCTG110600.034.7020531
TATAACG5450.033.65742
CCTGGGG86350.029.4680394
GTATCAA265250.028.548981
GGTATCA185800.028.1500531
TAACGCA7200.026.7835224
ATAACGC8050.025.124233
TCAACGC304550.024.5712874
GTACATG231400.024.513431
CAACGCA308250.024.2757075
ATCAACG308950.024.1454413
TATCAAC314700.023.7935262
AACGCAG317500.023.6301886
TACATGG236150.023.4821742
CATGGGG145850.023.3156834
ACATGGG237150.022.4910683
TATCACG5250.022.3971712
ATGGGGG92250.021.6684255
Produced by FastQC (version 0.11.5)