FastQCFastQC Report
Mon 6 Jun 2016
ERR523023_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523023_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5658173
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT203730.36006322182089523No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175820.31073634545992146No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96490.17053207811072585No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT68950.12185912307736083No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG87050.048.765242
ACCTGGG93750.044.1241043
TATAACG5100.038.714222
GTACCTG114400.037.501131
CCTGGGG86950.032.437414
GGTATCA176350.030.035751
GTATCAA251200.028.3144231
ATAACGC8000.026.4415573
GTACATG224750.025.4094071
TAACGCA7450.025.2387714
TACATGG229900.024.1696222
TCAACGC293700.023.8317134
CAACGCA296350.023.6188165
ATCAACG298150.023.4917813
CATGGGG146150.023.4151554
TATCAAC301150.023.3060282
ATGGGGG88250.023.2241675
ACATGGG230600.023.218223
AACGCAG306400.023.0552316
TGGGGGG87700.022.6158076
Produced by FastQC (version 0.11.5)