FastQCFastQC Report
Mon 6 Jun 2016
ERR523022_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523022_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8906296
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT289820.3254102491091695No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT221130.24828503341905545No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125870.14132698935674268No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA148300.048.450321
GTATCAA259250.039.440261
TACCTGG87050.034.610952
TCAACGC311350.032.4348224
ATCAACG316550.031.831733
CAACGCA319350.031.6077655
TATCAAC325300.031.0799672
AACGCAG332550.030.6025126
GTACCTG110400.028.2154961
GTACATG307750.027.536861
ACGCAGA374750.027.0440317
ACCTGGG111950.026.4480863
TACATGG316200.026.2068422
CGCAGAG390200.026.0083188
ACATGGG320750.024.8799113
TATAACG7400.024.13662
CATGGGG228900.023.3625624
GCAGAGT435400.023.0259519
ATGGGGG136450.021.4556165
GAGTACT248500.021.31646512-13
Produced by FastQC (version 0.11.5)