FastQCFastQC Report
Mon 6 Jun 2016
ERR523022_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523022_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8906296
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT260830.29286024178850556No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT216090.24262611527845018No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123420.13857612637172625No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA143500.047.44081
GTATCAA252450.039.713981
TACCTGG85850.037.5820162
TCAACGC307600.031.853164
ATCAACG311650.031.4404543
CAACGCA314050.031.1989545
TATCAAC319150.030.8292562
AACGCAG328700.029.9796566
GTACCTG113300.029.3994181
ACCTGGG110000.028.7095553
GTACATG300100.026.5415521
ACGCAGA375600.026.1734877
TACATGG308400.025.239452
CGCAGAG394050.024.9291938
ACATGGG313450.024.0933653
CATGGGG223400.022.907494
GCAGAGT434100.022.3800749
TATAACG9500.021.7833822
TATCACG9400.021.5147782
ATGGGGG133650.020.534155
Produced by FastQC (version 0.11.5)