FastQCFastQC Report
Mon 6 Jun 2016
ERR523021_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523021_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9807932
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT363450.3705674142112731No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT292740.29847270556117234No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154150.15716870793965537No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT124740.12718277410569323No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG109650.037.6152842
ACCTGGG128600.030.7194863
TATAACG8050.029.7952962
GTACCTG144250.029.4729611
GTATCAA476400.026.1209681
GTACATG375050.024.8283141
GGTATCA346700.024.0370431
ATAACGC11200.023.9349463
TACATGG385950.023.7982252
ACATGGG383350.023.2727363
TCAACGC542850.022.5854054
CATGGGG233950.022.4943664
CAACGCA547550.022.364525
ATCAACG550000.022.26663
TAACGCA10850.022.1058464
TATCAAC558550.022.0266822
AACGCAG562550.021.9348876
ATGGGGG145450.021.0479415
CCTGGGG129800.020.9420814
GAGTACT302750.020.7405812-13
Produced by FastQC (version 0.11.5)