FastQCFastQC Report
Mon 6 Jun 2016
ERR523021_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523021_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9807932
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT338390.3450166661024974No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT293770.29952287597426247No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155110.1581475075479724No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT127020.12950742317544617No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG118350.036.262082
ACCTGGG135200.030.7678283
TATAACG8450.029.4828432
GTACCTG150850.028.9688131
TAACGCA11350.025.6757934
GTATCAA485000.025.2460121
GGTATCA355000.024.8181691
ATAACGC11650.023.804353
GTACATG367500.023.7052211
TACATGG375150.022.7792362
TCAACGC546400.022.1682684
ACATGGG373200.022.0155753
CAACGCA551150.022.011445
CATGGGG225650.021.9341664
CCTGGGG128550.021.8288234
ATCAACG555050.021.8144383
AACGCAG567900.021.4013146
TATCAAC567150.021.399742
ATGGGGG142500.020.648565
GAGTACT316100.020.45868512-13
Produced by FastQC (version 0.11.5)