FastQCFastQC Report
Mon 6 Jun 2016
ERR523020_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523020_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7250409
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT327740.45202967170541686No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT299700.41335599136545265No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT267670.3691791732025049No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT157330.216994655060149No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131120.1808449702630569No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA100230.1382404771923901No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG116150.051.4956132
GTACCTG133950.045.4017221
ACCTGGG134950.043.7645153
CCTGGGG118950.032.806654
TATCACG7450.027.1402932
TATAACG7050.026.6792262
CTGGGGG76700.024.9472835
ATGGGGG122850.023.0380425
CATGGGG204400.022.9100864
ATAACGC9250.021.8590873
TGGGGGG136450.021.6282376
TAACGCA9100.021.1831594
GTACATG423000.020.8375071
GAGTACT261150.020.65664712-13
TACATGG433500.019.8827152
GTACACG18200.019.6407811
ACATGGG425900.019.4868853
AGTACTT276050.018.13734212-13
GTACTTT310450.017.32449714-15
TACACGG17100.015.9490282
Produced by FastQC (version 0.11.5)